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  • Polyethylenimine: An Intranasal Adjuvant for Liposomal Peptide-Based Subunit Vaccine against Group A Streptococcus

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    Embargoed until: 2021-08-07
    File version
    Accepted Manuscript (AM)
    Author(s)
    Dai, Charles C
    Yang, Jieru
    Hussein, Waleed M
    Zhao, Lili
    Wang, Xiumin
    Khalil, Zeinab G
    Capon, Robert J
    Toth, Istvan
    Stephenson, Rachel J
    Griffith University Author(s)
    Dai, Charles C.
    Year published
    2020
    Metadata
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    Abstract
    Group A Streptococcus (GAS) and GAS-related infections are a worldwide challenge, with no commercial GAS vaccine available. Polyethylenimine (PEI) attaches to the cells’ surface and delivers cargo into endosomal and cytosolic compartments. We hypothesized that this will confer mucosal adjuvant properties for peptide antigens against group A Streptococcus (GAS). In this study, we successfully demonstrated the development of PEI incorporated liposomes for the delivery of a lipopeptide-based vaccine (LCP-1) against GAS. Outbred mice were administrated with the vaccine formulations intranasally, and immunological investigation ...
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    Group A Streptococcus (GAS) and GAS-related infections are a worldwide challenge, with no commercial GAS vaccine available. Polyethylenimine (PEI) attaches to the cells’ surface and delivers cargo into endosomal and cytosolic compartments. We hypothesized that this will confer mucosal adjuvant properties for peptide antigens against group A Streptococcus (GAS). In this study, we successfully demonstrated the development of PEI incorporated liposomes for the delivery of a lipopeptide-based vaccine (LCP-1) against GAS. Outbred mice were administrated with the vaccine formulations intranasally, and immunological investigation showed that the PEI liposomes elicited significant mucosal and systemic immunity with the production of IgA and IgG antibodies. Antibodies were shown to effectively opsonize multiple isolates of clinically isolated GAS. This proof-of-concept study showed the capability for PEI liposomes to act as a safe vehicle for the delivery of GAS peptide antigens to elicit immune responses against GAS infection, making PEI a promising addition to liposomal mucosal vaccines.
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    Journal Title
    ACS Infectious Diseases
    Volume
    6
    Issue
    9
    DOI
    https://doi.org/10.1021/acsinfecdis.0c00452
    Copyright Statement
    This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Infectious Diseases, © YEAR American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://doi.org/10.1021/acsinfecdis.0c00452
    Subject
    Medical Microbiology
    Publication URI
    http://hdl.handle.net/10072/402737
    Collection
    • Journal articles

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