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dc.contributor.authorVadivelu, R
dc.contributor.authorKashaninejad, N
dc.contributor.authorNikmaneshi, MR
dc.contributor.authorKhadim, RR
dc.contributor.authorSalehi, SS
dc.contributor.authorRamulu, NC
dc.contributor.authorSakai, Y
dc.contributor.authorNishikawa, M
dc.contributor.authorFiroozabadi, B
dc.contributor.authorNguyen, NT
dc.date.accessioned2021-03-04T04:28:44Z
dc.date.available2021-03-04T04:28:44Z
dc.date.issued2021
dc.identifier.issn2701-0198
dc.identifier.doi10.1002/adbi.202000108
dc.identifier.urihttp://hdl.handle.net/10072/402775
dc.description.abstractDigital microfluidics based on liquid marble (LM) has recently emerged as a promising platform for liquid handling and cell-based assays. However, evaporation is a critical problem in such platforms, hindering their wide-range applications in various fields. This study aims to develop a functional sessile LM system for long-term 3D cell culture. Previously, this study group and others demonstrated that floating LM-based bioreactors could reduce the evaporation rate, and were thus suitable for growing multicellular spheroids. However, floating LMs are not robust and easily collapse. Herein, an evaporation-reducing sessile LM by embedding LM with agarose gel is proposed. Through a series of comprehensive mathematical modeling, numerical simulations, and experimental investigations (both with and without biological cells), it is shown that such a platform acts as a moisture absorption system to control the evaporation and thus extends the life span of LMs. It is also found that unlike pure LMs, the LMs filled with agarose maintain their spherical shapes within 72 h inside a humidified incubator. Moreover, the presence of agarose significantly contributes to minimizing evaporation and improves the viability of the harvested multicellular spheroids. These results can open up a new avenue in using LMs in life sciences and chemistry.
dc.description.peerreviewedYes
dc.languageen
dc.publisherWiley
dc.relation.ispartofpagefrom2000108
dc.relation.ispartofissue2
dc.relation.ispartofjournalAdvanced Biology
dc.relation.ispartofvolume5
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchNanotechnology
dc.subject.fieldofresearchcode3101
dc.subject.fieldofresearchcode4018
dc.titleSessile Liquid Marbles with Embedded Hydrogels as Bioreactors for Three-Dimensional Cell Culture
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationVadivelu, R; Kashaninejad, N; Nikmaneshi, MR; Khadim, RR; Salehi, SS; Ramulu, NC; Sakai, Y; Nishikawa, M; Firoozabadi, B; Nguyen, NT, Sessile Liquid Marbles with Embedded Hydrogels as Bioreactors for Three-Dimensional Cell Culture, Advanced Biology, 2021, 5 (2), pp. 2000108
dc.date.updated2021-03-04T00:48:14Z
gro.description.notepublicThis publication has been entered as an advanced online version in Griffith Research Online.
gro.hasfulltextNo Full Text
gro.griffith.authorKashaninejad, Navid
gro.griffith.authorNguyen, Nam-Trung
gro.griffith.authorVadivelu, Raja


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