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dc.contributor.authorDai, C
dc.contributor.authorStephenson, RJ
dc.contributor.authorSkwarczynski, M
dc.contributor.authorToth, I
dc.contributor.editorHussein, Waleed M
dc.contributor.editorSkwarczynski, Mariusz
dc.contributor.editorToth, Istvan
dc.date.accessioned2021-03-08T21:37:03Z
dc.date.available2021-03-08T21:37:03Z
dc.date.issued2020
dc.identifier.isbn978-1-0716-0226-3
dc.identifier.doi10.1007/978-1-0716-0227-0_2
dc.identifier.urihttp://hdl.handle.net/10072/402791
dc.description.abstractFmoc solid-phase peptide synthesis (SPPS) is the most common approach used to synthesize natural and unnatural peptides. However, the synthesis of sequences longer than 30–60 amino acids is associated with a drastic reduction in peptide quality. Thus, large and complex peptides are normally synthesized as fragments, which are then conjugated together. Here, we describe the synthesis of a large, branched peptide, a multi-epitope vaccine candidate against Group A Streptococcus, with the help of microwave-assisted Fmoc-SPPS, thiol-maleimide conjugation, and copper(I)-catalyzed alkyne-azide cycloaddition (CuAAC) “click” reaction.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherSpringer
dc.publisher.placeNew York, United States
dc.relation.ispartofbooktitlePeptide Synethesis: Methods and Protocols
dc.relation.ispartofchapter2
dc.relation.ispartofchapternumbers22
dc.relation.ispartofpagefrom13
dc.relation.ispartofpageto27
dc.relation.ispartofseriesMethods in Molecular Biology
dc.relation.ispartofvolume2103
dc.subject.fieldofresearchOther Chemical Sciences
dc.subject.fieldofresearchBiochemistry and Cell Biology
dc.subject.fieldofresearchcode0399
dc.subject.fieldofresearchcode0601
dc.subject.keywordsBranched peptide synthesis
dc.subject.keywordsCopper-catalyzed azide-alkyne cycloaddition click reaction
dc.subject.keywordsFmoc solid-phase peptide synthesis
dc.subject.keywordsGroup A Streptococcus
dc.subject.keywordsMicrowave-assisted SPPS
dc.titleApplication of Fmoc-SPPS, Thiol-Maleimide Conjugation, and Copper(i)-Catalyzed Alkyne-Aazide Cycloaddition “Click” Reaction in the Synthesis of a Complex Peptide-Based Vaccine Candidate Against Group A Streptococcus
dc.typeBook chapter
dc.type.descriptionB1 - Chapters
dcterms.bibliographicCitationDai, C; Stephenson, RJ; Skwarczynski, M; Toth, I, Application of Fmoc-SPPS, Thiol-Maleimide Conjugation, and Copper(i)-Catalyzed Alkyne-Aazide Cycloaddition “Click” Reaction in the Synthesis of a Complex Peptide-Based Vaccine Candidate Against Group A Streptococcus, Peptide Synethesis: Methods and Protocols, 2020, 2103, pp. 13-27
dc.date.updated2021-03-03T04:37:51Z
dc.description.versionAccepted Manuscript (AM)
gro.rights.copyright© 2020 Springer. This is the author-manuscript version of this paper. It is reproduced here in accordance with the copyright policy of the publisher. Please refer to the publisher’s website for further information.
gro.hasfulltextFull Text
gro.griffith.authorDai, Charles C.


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