dc.contributor.author | Dai, C | |
dc.contributor.author | Stephenson, RJ | |
dc.contributor.author | Skwarczynski, M | |
dc.contributor.author | Toth, I | |
dc.contributor.editor | Hussein, Waleed M | |
dc.contributor.editor | Skwarczynski, Mariusz | |
dc.contributor.editor | Toth, Istvan | |
dc.date.accessioned | 2021-03-08T21:37:03Z | |
dc.date.available | 2021-03-08T21:37:03Z | |
dc.date.issued | 2020 | |
dc.identifier.isbn | 978-1-0716-0226-3 | |
dc.identifier.doi | 10.1007/978-1-0716-0227-0_2 | |
dc.identifier.uri | http://hdl.handle.net/10072/402791 | |
dc.description.abstract | Fmoc solid-phase peptide synthesis (SPPS) is the most common approach used to synthesize natural and unnatural peptides. However, the synthesis of sequences longer than 30–60 amino acids is associated with a drastic reduction in peptide quality. Thus, large and complex peptides are normally synthesized as fragments, which are then conjugated together. Here, we describe the synthesis of a large, branched peptide, a multi-epitope vaccine candidate against Group A Streptococcus, with the help of microwave-assisted Fmoc-SPPS, thiol-maleimide conjugation, and copper(I)-catalyzed alkyne-azide cycloaddition (CuAAC) “click” reaction. | |
dc.description.peerreviewed | Yes | |
dc.language | English | |
dc.publisher | Springer | |
dc.publisher.place | New York, United States | |
dc.relation.ispartofbooktitle | Peptide Synethesis: Methods and Protocols | |
dc.relation.ispartofchapter | 2 | |
dc.relation.ispartofchapternumbers | 22 | |
dc.relation.ispartofpagefrom | 13 | |
dc.relation.ispartofpageto | 27 | |
dc.relation.ispartofseries | Methods in Molecular Biology | |
dc.relation.ispartofvolume | 2103 | |
dc.subject.fieldofresearch | Other Chemical Sciences | |
dc.subject.fieldofresearch | Biochemistry and Cell Biology | |
dc.subject.fieldofresearchcode | 0399 | |
dc.subject.fieldofresearchcode | 0601 | |
dc.subject.keywords | Branched peptide synthesis | |
dc.subject.keywords | Copper-catalyzed azide-alkyne cycloaddition click reaction | |
dc.subject.keywords | Fmoc solid-phase peptide synthesis | |
dc.subject.keywords | Group A Streptococcus | |
dc.subject.keywords | Microwave-assisted SPPS | |
dc.title | Application of Fmoc-SPPS, Thiol-Maleimide Conjugation, and Copper(i)-Catalyzed Alkyne-Aazide Cycloaddition “Click” Reaction in the Synthesis of a Complex Peptide-Based Vaccine Candidate Against Group A Streptococcus | |
dc.type | Book chapter | |
dc.type.description | B1 - Chapters | |
dcterms.bibliographicCitation | Dai, C; Stephenson, RJ; Skwarczynski, M; Toth, I, Application of Fmoc-SPPS, Thiol-Maleimide Conjugation, and Copper(i)-Catalyzed Alkyne-Aazide Cycloaddition “Click” Reaction in the Synthesis of a Complex Peptide-Based Vaccine Candidate Against Group A Streptococcus, Peptide Synethesis: Methods and Protocols, 2020, 2103, pp. 13-27 | |
dc.date.updated | 2021-03-03T04:37:51Z | |
dc.description.version | Accepted Manuscript (AM) | |
gro.rights.copyright | © 2020 Springer. This is the author-manuscript version of this paper. It is reproduced here in accordance with the copyright policy of the publisher. Please refer to the publisher’s website for further information. | |
gro.hasfulltext | Full Text | |
gro.griffith.author | Dai, Charles C. | |