Show simple item record

dc.contributor.authorDai, Charles C
dc.contributor.authorHuang, Wenbin
dc.contributor.authorYang, Jieru
dc.contributor.authorHussein, Waleed M
dc.contributor.authorWang, Jingwen
dc.contributor.authorKhalil, Zeinab G
dc.contributor.authorCapon, Robert J
dc.contributor.authorToth, Istvan
dc.contributor.authorStephenson, Rachel J
dc.date.accessioned2021-03-10T00:51:33Z
dc.date.available2021-03-10T00:51:33Z
dc.date.issued2021
dc.identifier.issn0960-894X
dc.identifier.doi10.1016/j.bmcl.2021.127920
dc.identifier.urihttp://hdl.handle.net/10072/403029
dc.description.abstractWe recently reported that polyethylenimine (PEI; molecular weight of 600 Da) acted as a vaccine adjuvant for liposomal group A Streptococcus (GAS) vaccines, eliciting immune responses in vivo with IgG antibodies giving opsonic activity against five Australian GAS clinical isolates. However, to date, no investigation comparing the structure-activity relationship between the molecular weight of PEI and its adjuvanting activity in vaccine development has been performed. We hypothesized that the molecular weight and quantity of PEI in a liposomal vaccine will impact its adjuvant properties. In this study, we successfully formulated liposomes containing different molecular weights of PEI (600, 1800, 10k and 25k Da) and equivalents of PEI (0.5, 1 and 2) of branched PEI. Outbred mice were administrated the vaccine formulations intranasally, and the mice that received a high ratio of PEI 600 reported a stronger immune response than the mice that received a lower ratio of PEI 600. Interestingly, mice that received the same quantity of PEI 600, PEI 10k and PEI 25k showed similar immune responses in vivo and in vitro. This comparative study highlights the ratio of PEI present in the liposome vaccines impacts adjuvant activity, however, PEI molecular weight did not significantly enhance its adjuvant properties. We also report that the stability of PEI liposomes is critical for vaccines to elicit the desired immune response.
dc.description.peerreviewedYes
dc.languageen
dc.publisherElsevier BV
dc.relation.ispartofjournalBioorganic & Medicinal Chemistry Letters
dc.subject.fieldofresearchMedicinal and biomolecular chemistry
dc.subject.fieldofresearchOrganic chemistry
dc.subject.fieldofresearchPharmacology and pharmaceutical sciences
dc.subject.fieldofresearchcode3404
dc.subject.fieldofresearchcode3405
dc.subject.fieldofresearchcode3214
dc.titlePolyethylenimine quantity and molecular weight influence its adjuvanting properties in liposomal peptide vaccines
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationDai, CC; Huang, W; Yang, J; Hussein, WM; Wang, J; Khalil, ZG; Capon, RJ; Toth, I; Stephenson, RJ, Polyethylenimine quantity and molecular weight influence its adjuvanting properties in liposomal peptide vaccines, Bioorganic & Medicinal Chemistry Letters, 2021
dcterms.licensehttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.date.updated2021-03-10T00:44:09Z
dc.description.versionAccepted Manuscript (AM)
gro.description.notepublicThis publication has been entered in Griffith Research Online as an advanced online version.
gro.rights.copyright© 2021 Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence (http://creativecommons.org/licenses/by-nc-nd/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorDai, Charles C.


Files in this item

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record