dc.contributor.author | Budiono, Boris P | |
dc.contributor.author | See Hoe, Louise E | |
dc.contributor.author | Peart, Jason N | |
dc.contributor.author | Vider, Jelena | |
dc.contributor.author | Ashton, Kevin J | |
dc.contributor.author | Jacques, Angela | |
dc.contributor.author | Haseler, Luke J | |
dc.contributor.author | Headrick, John P | |
dc.date.accessioned | 2021-03-12T03:34:08Z | |
dc.date.available | 2021-03-12T03:34:08Z | |
dc.date.issued | 2021 | |
dc.identifier.issn | 0024-3205 | |
dc.identifier.doi | 10.1016/j.lfs.2021.119253 | |
dc.identifier.uri | http://hdl.handle.net/10072/403111 | |
dc.description.abstract | AIM: Exercise is cardioprotective, though optimal interventions are unclear. We assessed duration dependent effects of exercise on myocardial ischemia-reperfusion (I-R) injury, kinase signaling and gene expression. METHODS: Responses to brief (2 day; 2EX), intermediate (7 and 14 day; 7EX and 14EX) and extended (28 day; 28EX) voluntary wheel running (VWR) were studied in male C57Bl/6 mice. Cardiac function, I-R tolerance and survival kinase signaling were assessed in perfused hearts. KEY FINDINGS: Mice progressively increased running distances and intensity, from 2.4 ± 0.2 km/day (0.55 ± 0.04 m/s) at 2-days to 10.6 ± 0.4 km/day (0.72 ± 0.06 m/s) after 28-days. Myocardial mass and contractility were modified at 14-28 days VWR. Cardioprotection was not 'dose-dependent', with I-R tolerance enhanced within 7 days and not further improved with greater VWR duration, volume or intensity. Protection was associated with AKT, ERK1/2 and GSK3β phosphorylation, with phospho-AMPK selectively enhanced with brief VWR. Gene expression was duration-dependent: 7 day VWR up-regulated glycolytic (Pfkm) and down-regulated maladaptive remodeling (Mmp2) genes; 28 day VWR up-regulated caveolar (Cav3), mitochondrial biogenesis (Ppargc1a, Sirt3) and titin (Ttn) genes. Interestingly, I-R tolerance in 2EX/2SED groups improved vs. groups subjected to longer sedentariness, suggesting transient protection on transition to housing with running wheels. SIGNIFICANCE: Cardioprotection is induced with as little as 7 days VWR, yet not enhanced with further or faster running. This protection is linked to survival kinase phospho-regulation (particularly AKT and ERK1/2), with glycolytic, mitochondrial, caveolar and myofibrillar gene changes potentially contributing. Intriguingly, environmental enrichment may also protect via similar kinase regulation. | |
dc.description.peerreviewed | Yes | |
dc.language | eng | |
dc.publisher | Elsevier BV | |
dc.relation.ispartofjournal | Life sciences | |
dc.subject.fieldofresearch | Biochemistry and cell biology | |
dc.subject.fieldofresearch | Pharmacology and pharmaceutical sciences | |
dc.subject.fieldofresearchcode | 3101 | |
dc.subject.fieldofresearchcode | 3214 | |
dc.subject.keywords | Cardioprotection | |
dc.subject.keywords | Exercise | |
dc.subject.keywords | Myocardial ischemia | |
dc.subject.keywords | Survival kinase | |
dc.subject.keywords | Voluntary activity | |
dc.title | Effects of voluntary exercise duration on myocardial ischaemic tolerance, kinase signaling and gene expression | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dcterms.bibliographicCitation | Budiono, BP; See Hoe, LE; Peart, JN; Vider, J; Ashton, KJ; Jacques, A; Haseler, LJ; Headrick, JP, Effects of voluntary exercise duration on myocardial ischaemic tolerance, kinase signaling and gene expression, Life sciences, 2021 | |
dcterms.dateAccepted | 2021-02-17 | |
dcterms.license | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.date.updated | 2021-03-12T02:58:51Z | |
dc.description.version | Accepted Manuscript (AM) | |
gro.description.notepublic | This publication has been entered in Griffith Research Online as an advanced online version. | |
gro.rights.copyright | © 2021 Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence (http://creativecommons.org/licenses/by-nc-nd/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited. | |
gro.hasfulltext | Full Text | |
gro.griffith.author | Headrick, John P. | |
gro.griffith.author | Peart, Jason N. | |