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  • Carcinoembryonic antigen glycosylation: revealing novel features of human glycosylation and cancer origin specificity

    Author(s)
    Almeida, Andreia
    Jacob, Francis
    Stavenhagen, Kathrin
    Alagesan, Kathrivel
    Mischak, Michaela
    Wuhrer, Manfred
    Everest-Dass, Arunu
    Reis, Celso A
    Kolarich, Daniel
    Griffith University Author(s)
    Kolarich, Daniel
    Alagesan, Kathirvel
    Everest-Dass, Arun
    Year published
    2020
    Metadata
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    Abstract
    Carcinoembryonic Antigen (CEA) is a biomarker strongly associated with tumour progression and metastasis. Even though N-glycans make up »50% of the entire CEA molecule, current knowledge on CEA specific glycosylation in health and disease is scarce. We show for the first time in an indepth glycomics and glycoproteomics study that the over 270 different N-glycans identified exhibited antenna fucosylation and sialylation features that allowed a clear CEA body origin assignment. Colon-derived CEA carried a hitherto not described, hexosylated bisected GlcNAc glycoepitope. All analysed CEAs contained a 29th site of N-glycosylation ...
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    Carcinoembryonic Antigen (CEA) is a biomarker strongly associated with tumour progression and metastasis. Even though N-glycans make up »50% of the entire CEA molecule, current knowledge on CEA specific glycosylation in health and disease is scarce. We show for the first time in an indepth glycomics and glycoproteomics study that the over 270 different N-glycans identified exhibited antenna fucosylation and sialylation features that allowed a clear CEA body origin assignment. Colon-derived CEA carried a hitherto not described, hexosylated bisected GlcNAc glycoepitope. All analysed CEAs contained a 29th site of N-glycosylation on Asn71, located within a non-canonical 71N-R-Q73 sequence motif critical for CD8a binding. Correlation analyses of CEA and glycosyltransferase genes across the TGCA dataset revealed that CEACAM5 and B4GALNT3 expression levels were indicative for survival prediction. Our results open novel opportunities to understand CEA function, its role as a cancer marker but also reveal hitherto unknown aspects of glycobiology
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    Conference Title
    Glycobiology
    Volume
    30
    Issue
    12
    Publisher URI
    https://academic.oup.com/glycob/article/30/12/1016/5948902
    Subject
    Biological sciences
    Biomedical and clinical sciences
    Clinical sciences
    Oncology and carcinogenesis
    Science & Technology
    Life Sciences & Biomedicine
    Biochemistry & Molecular Biology
    Publication URI
    http://hdl.handle.net/10072/403273
    Collection
    • Conference outputs

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