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dc.contributor.authorMcKeating, Daniel
dc.contributor.authorBennett, William
dc.contributor.authorVanerlelie, Jessica
dc.contributor.authorPerkins, Anthony
dc.date.accessioned2021-03-18T06:45:03Z
dc.date.available2021-03-18T06:45:03Z
dc.date.issued2018
dc.identifier.issn0143-4004
dc.identifier.urihttp://hdl.handle.net/10072/403277
dc.description.abstractObjectives: Aberrant micronutrient deficiencies are associated with arange of perinatal outcomes and concomitant with gestational disorderssuch as: gestational diabetes mellitus; preeclampsia; and an increased riskof foetal growth restriction, preterm birth and stillbirth. This project aimsto develop a comprehensive method to determine concentrations ofessential micronutrients in a single blood sample; ultimately describingthe individual’s micronutrient profile, and aiming to identify women atrisk of complications. Methods: Inductively coupled plasma mass spectrometry (ICP-MS) wasused to simultaneous measure elements in samples of serum, plasmaand whole blood. For primary methodology optimisation, cord bloodsamples (n¼9) were collected from the Gold Coast UniversityHospital, Australia. Two ICP-MS sample preparation methodologies weretrialled: dilution with (1) 2% nitric acid (HNO3), and (2) a solution ofEDTA and Ammonia. Testing involved determining dilution factors,specificity, intra-assay variation and inter-assay variation on 69 ele-ments. For secondary determination of elements associated with preg-nancy complications, cord blood samples from the Environments forHealthy Living (EFHL) cohort (n¼160) were screened with the optimisedmethod. Results: EDTA-based preparation was used due to significantly lowerintra- and inter-assay variation for sera, plasma, and whole blood samples.Furthermore, it had significantly higher specificity for the majority of el-ements, allowing for increased distinction between elemental concentra-tions (p<0.05). In the EFHL samples, 40 elements were able to be analysed,however 13 were excluded due to higher than 10% variation within trip-licates; 27 elements were determined to be highly reproducible (<10%variation) in this simultaneous analysis. Conclusion: This study is thefirst to analyse 27 elements in pregnancysamples. Hallmark elements measured include: sodium, magnesium, po-tassium, calcium, manganese, iron, zinc, selenium, molybdenum, lead andiodine. The number of quantifiable micronutrients could provide immensestatistical power for analysis of gestational disorders, providing insightinto ways to minimise disease outcomes through supplemental micro-nutrition
dc.languageEnglish
dc.publisherElsevier
dc.publisher.urihttps://www.sciencedirect.com/science/article/pii/S014340041830290X
dc.relation.ispartofconferencenameMeeting of the International-Federation-of-Placenta-Associations (IFPA)
dc.relation.ispartofconferencetitlePlacenta
dc.relation.ispartofdatefrom2018-09-21
dc.relation.ispartofdateto2018-09-24
dc.relation.ispartoflocationTokyo, Japan
dc.relation.ispartofpagefromE34
dc.relation.ispartofpagetoE34
dc.relation.ispartofvolume69
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchClinical sciences
dc.subject.fieldofresearchReproductive medicine
dc.subject.fieldofresearchMidwifery
dc.subject.fieldofresearchcode3101
dc.subject.fieldofresearchcode3202
dc.subject.fieldofresearchcode3215
dc.subject.fieldofresearchcode4204
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsDevelopmental Biology
dc.subject.keywordsObstetrics & Gynecology
dc.subject.keywordsReproductive Biology
dc.titleElemental analysis for the determination of micronutrient status in biological samples: Applications for pregnancy research
dc.typeConference output
dc.type.descriptionE3 - Conferences (Extract Paper)
dcterms.bibliographicCitationMcKeating, D; Bennett, W; Vanerlelie, J; Perkins, A, Elemental analysis for the determination of micronutrient status in biological samples: Applications for pregnancy research, Placenta, 2018, 69, pp. E34-E34
dc.date.updated2021-03-18T06:41:58Z
gro.hasfulltextNo Full Text
gro.griffith.authorMcKeating, Daniel R.
gro.griffith.authorBennett, Will W.


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