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  • Evaluation of bacterial kill in human roots infected with Enterococcus faecalis biofilm following 980 nm laser activation of chlorhexidine

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    Love475904-Published.pdf (6.551Mb)
    File version
    Version of Record (VoR)
    Author(s)
    Jiang, Anna P
    Ipe, Deepak S
    Love, Robert M
    George, Roy
    Griffith University Author(s)
    Love, Robert M.
    George, Roy
    Ipe, Deepak S.
    Jiang, Anna
    Year published
    2020
    Metadata
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    Abstract
    Effectiveness of root canal disinfection greatly determines the success of endodontic treatment. The purpose of this study was to evaluate the bacterial killing efficacy of chlorhexidine (CHX) activated with a Gemni 980 nm diode laser. Bacterial kill at the apical one third of the endodontically prepared roots were assessed using the 3D fluorescent microscope (SZX16) after staining the tooth with BacLight SYTO 9 (Live/Dead). The percentage of bacterial kill was calculated by measuring the total amount of green pixels (live bacteria) and red pixels (dead bacteria) using the Image color summarizer image color statistics and ...
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    Effectiveness of root canal disinfection greatly determines the success of endodontic treatment. The purpose of this study was to evaluate the bacterial killing efficacy of chlorhexidine (CHX) activated with a Gemni 980 nm diode laser. Bacterial kill at the apical one third of the endodontically prepared roots were assessed using the 3D fluorescent microscope (SZX16) after staining the tooth with BacLight SYTO 9 (Live/Dead). The percentage of bacterial kill was calculated by measuring the total amount of green pixels (live bacteria) and red pixels (dead bacteria) using the Image color summarizer image color statistics and clustering program (Circos—Circular Genome Visualization/Martin Krzywinski). The 3D fluorescent ex‐vivo tooth model allowed real time measurement of the percentage of bacterial kill at specified time points by capturing the bacterial kill on the external surface of the root, hence indicating the amount of penetration of the irrigation solution through the radicular dentine. Compared to CHX alone, both Endoactivator and diode laser treatment groups showed significantly better bacterial kill following a 72‐hour study period (P > .05). However, of clinical importance is the larger number of bacteria killed with laser assisted irrigation immediately following treatment when compared to the use of CHX alone.image
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    Journal Title
    Translational Biophotonics
    Volume
    2
    Issue
    4
    DOI
    https://doi.org/10.1002/tbio.202000008
    Copyright Statement
    © 2020 The Authors. Translational Biophotonics published by Wiley‐VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
    Subject
    Dentistry
    Publication URI
    http://hdl.handle.net/10072/403444
    Collection
    • Journal articles

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