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  • Salivary bacterial shifts in oral leukoplakia resemble the dysbiotic oral cancer bacteriome

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    Johnson456087-Published.pdf (3.626Mb)
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    Author(s)
    Gopinath, Divya
    Menon, Rohit Kunnath
    Wie, Chong Chun
    Banerjee, Moinak
    Panda, Swagatika
    Mandal, Deviprasad
    Behera, Paresh Kumar
    Roychoudhury, Susanta
    Kheur, Supriya
    Botelho, Michael George
    Johnson, Newell W
    Griffith University Author(s)
    Johnson, Newell W.
    Year published
    2021
    Metadata
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    Abstract
    Objective: While some oral carcinomas appear to arise de novo, others develop within long-standing conditions of the oral cavity that have malignant potential, now known as oral potentially malignant disorders (OPMDs). The oral bacteriome associated with OPMD has been studied to a lesser extent than that associated with oral cancer. To characterize the association in detail we compared the bacteriome in whole mouth fluid (WMF) in patients with oral leukoplakia, oral cancer and healthy controls. Methods: WMF bacteriome from 20 leukoplakia patients, 31 patients with oral cancer and 23 healthy controls were profiled using the ...
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    Objective: While some oral carcinomas appear to arise de novo, others develop within long-standing conditions of the oral cavity that have malignant potential, now known as oral potentially malignant disorders (OPMDs). The oral bacteriome associated with OPMD has been studied to a lesser extent than that associated with oral cancer. To characterize the association in detail we compared the bacteriome in whole mouth fluid (WMF) in patients with oral leukoplakia, oral cancer and healthy controls. Methods: WMF bacteriome from 20 leukoplakia patients, 31 patients with oral cancer and 23 healthy controls were profiled using the Illumina MiSeq platform. Sequencing reads were processed using DADA2, and taxonomical classification was performed using the phylogenetic placement method. Sparse Partial Least Squares Regression Discriminant Analysis model was used to identify bacterial taxa that best discriminate the studied groups. Results: We found considerable overlap between the WMF bacteriome of leukoplakia and oral cancer while a clearer separation between healthy controls and the former two disorders was observed. Specifically, the separation was attributed to 14 taxa belonging to the genera Megaspheara, unclassified enterobacteria, Prevotella, Porphyromonas, Rothia and Salmonella, Streptococcus, and Fusobacterium. The most discriminative bacterial genera between leukoplakia and oral cancer were Megasphaera, unclassified Enterobacteriae, Salmonella and Prevotella. Conclusion: Oral bacteria may play a role in the early stages of oral carcinogenesis as a dysbiotic bacteriome is associated with oral leukoplakia and this resembles that of oral cancer more than healthy controls. Our findings may have implications for developing oral cancer prevention strategies targeting early microbial drivers of oral carcinogenesis.
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    Journal Title
    Journal of Oral Microbiology
    Volume
    13
    Issue
    1
    DOI
    https://doi.org/10.1080/20002297.2020.1857998
    Copyright Statement
    © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
    Subject
    Medical microbiology
    Science & Technology
    Life Sciences & Biomedicine
    Microbiology
    Saliva
    bacteriome
    Publication URI
    http://hdl.handle.net/10072/404443
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    • Journal articles

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