Show simple item record

dc.contributor.authorKlionsky, Daniel J
dc.contributor.authorAbdel-Aziz, Amal Kamal
dc.contributor.authorAbdelfatah, Sara
dc.contributor.authorAbdellatif, Mahmoud
dc.contributor.authorAbdoli, Asghar
dc.contributor.authorAbel, Steffen
dc.contributor.authorAbeliovich, Hagai
dc.contributor.authorAbildgaard, Marie H.
dc.contributor.authorAbudu, Yakubu Princely
dc.contributor.authorAcevedo-Arozena, Abraham
dc.contributor.authorAdamopoulos, Iannis E.
dc.contributor.authorAdeli, Khosrow
dc.contributor.authorAdolph, Timon E.
dc.contributor.authorDharmasivam, Mahendiran
dc.contributor.authoret al.
dc.date.accessioned2021-05-18T23:50:22Z
dc.date.available2021-05-18T23:50:22Z
dc.date.issued2021
dc.identifier.issn1554-8627
dc.identifier.doi10.1080/15548627.2020.1797280
dc.identifier.urihttp://hdl.handle.net/10072/404489
dc.description.abstractIn 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherTaylor & Francis Inc
dc.relation.ispartofpagefrom1
dc.relation.ispartofpageto382
dc.relation.ispartofissue1
dc.relation.ispartofjournalAutophagy
dc.relation.ispartofvolume17
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchcode3101
dc.titleGuidelines for the use and interpretation of assays for monitoring autophagy (4th edition)
dc.typeJournal article
dc.type.descriptionC1 - Articles
gro.hasfulltextNo Full Text
gro.griffith.authorDharmasivam, Mahendiran
gro.griffith.authorXiao, Yin


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record