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dc.contributor.authorMoh, ESX
dc.contributor.authorPacker, NH
dc.date.accessioned2021-05-25T01:12:24Z
dc.date.available2021-05-25T01:12:24Z
dc.date.issued2021
dc.identifier.issn1525-7797
dc.identifier.doi10.1021/acs.biomac.0c01791
dc.identifier.urihttp://hdl.handle.net/10072/404630
dc.description.abstractSilk is a popular protein biomaterial that has been used for various purposes such as tissue scaffolding, textiles and hydrogels. Various methods for covalent conjugation of functional molecules such as small molecule sensors and enzymes have been developed to create functionalized silk biomaterials. Here, we report a method for silk functionalization by using O-GalNAc-transferases and azide-modified UDP-GalNAc nucleotide sugar substrates to incorporate azide functional groups onto the silk fibroin protein for functionalization with cycloalkynes by click chemistry. Using ppGalNAc-T1 and T13 enzymes, we could transfer azide-modified GalNAc monosaccharides onto fibroin and as a proof of concept, conjugated a strain-alkyne-functionalized Cy5 fluorophore to produce a Cy5-conjugated fibroin hydrogel. This facile azido functionalization of the silk has the potential for attachment of any cycloalkyne moiety.
dc.description.peerreviewedYes
dc.languageeng
dc.publisherAmerican Chemical Society (ACS)
dc.relation.ispartofpagefrom1752
dc.relation.ispartofpageto1755
dc.relation.ispartofissue4
dc.relation.ispartofjournalBiomacromolecules
dc.relation.ispartofvolume22
dc.subject.fieldofresearchChemical sciences
dc.subject.fieldofresearchMedicinal and biomolecular chemistry
dc.subject.fieldofresearchBiological sciences
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchEngineering
dc.subject.fieldofresearchcode34
dc.subject.fieldofresearchcode3404
dc.subject.fieldofresearchcode31
dc.subject.fieldofresearchcode3101
dc.subject.fieldofresearchcode40
dc.titleEnzymatic Azido-GalNAc-Functionalized Silk Fibroin for Click Chemistry Conjugation
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationMoh, ESX; Packer, NH, Enzymatic Azido-GalNAc-Functionalized Silk Fibroin for Click Chemistry Conjugation, Biomacromolecules, 2021, 22 (4), pp. 1752-1755
dc.date.updated2021-05-24T22:52:10Z
gro.hasfulltextNo Full Text
gro.griffith.authorPacker, Nicki


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