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  • Separation of distinct exosome subpopulations: isolation and characterization approaches and their associated challenges

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    Shiddiky493820-Accepted.pdf (2.066Mb)
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    Accepted Manuscript (AM)
    Author(s)
    Singh, Karishma
    Nalabotala, Ruchika
    Koo, Kevin M
    Bose, Sudeep
    Nayak, Ranu
    Shiddiky, Muhammad JA
    Griffith University Author(s)
    Shiddiky, Muhammad J.
    Year published
    2021
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    Abstract
    Exosomes are nano-sized extracellular vesicles that serve as a communications system between cells and have shown tremendous promise as liquid biopsy biomarkers in diagnostic, prognostic, and even therapeutic use in different human diseases. Due to the natural heterogeneity of exosomes, there is a need to separate exosomes into distinct biophysical and/or biochemical subpopulations to enable full interrogation of exosome biology and function prior to the possibility of clinical translation. Currently, there exists a multitude of different exosome isolation and characterization approaches which can, in limited capacity, ...
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    Exosomes are nano-sized extracellular vesicles that serve as a communications system between cells and have shown tremendous promise as liquid biopsy biomarkers in diagnostic, prognostic, and even therapeutic use in different human diseases. Due to the natural heterogeneity of exosomes, there is a need to separate exosomes into distinct biophysical and/or biochemical subpopulations to enable full interrogation of exosome biology and function prior to the possibility of clinical translation. Currently, there exists a multitude of different exosome isolation and characterization approaches which can, in limited capacity, separate exosomes based on biophysical and/or biochemical characteristics. While notable reviews in recent years have reviewed these approaches for bulk exosome sorting, we herein present a comprehensive overview of various conventional technologies and modern microfluidic and nanotechnological advancements towards isolation and characterization of exosome subpopulations. The benefits and limitations of these different technologies to improve their use for distinct exosome subpopulations in clinical practices are also discussed. Furthermore, an overview of the most commonly encountered technical and biological challenges for effective separation of exosome subpopulations is presented.
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    Journal Title
    Analyst
    DOI
    https://doi.org/10.1039/d1an00024a
    Copyright Statement
    © 2021 Royal Society of Chemistry. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal website for access to the definitive, published version.
    Note
    This publication has been entered in Griffith Research Online as an advanced online version.
    Subject
    Analytical chemistry
    Other chemical sciences
    Publication URI
    http://hdl.handle.net/10072/404640
    Collection
    • Journal articles

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