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dc.contributor.authorSingh, Karishma
dc.contributor.authorNalabotala, Ruchika
dc.contributor.authorKoo, Kevin M
dc.contributor.authorBose, Sudeep
dc.contributor.authorNayak, Ranu
dc.contributor.authorShiddiky, Muhammad JA
dc.date.accessioned2021-05-26T00:27:25Z
dc.date.available2021-05-26T00:27:25Z
dc.date.issued2021
dc.identifier.issn0003-2654en_US
dc.identifier.doi10.1039/d1an00024aen_US
dc.identifier.urihttp://hdl.handle.net/10072/404640
dc.description.abstractExosomes are nano-sized extracellular vesicles that serve as a communications system between cells and have shown tremendous promise as liquid biopsy biomarkers in diagnostic, prognostic, and even therapeutic use in different human diseases. Due to the natural heterogeneity of exosomes, there is a need to separate exosomes into distinct biophysical and/or biochemical subpopulations to enable full interrogation of exosome biology and function prior to the possibility of clinical translation. Currently, there exists a multitude of different exosome isolation and characterization approaches which can, in limited capacity, separate exosomes based on biophysical and/or biochemical characteristics. While notable reviews in recent years have reviewed these approaches for bulk exosome sorting, we herein present a comprehensive overview of various conventional technologies and modern microfluidic and nanotechnological advancements towards isolation and characterization of exosome subpopulations. The benefits and limitations of these different technologies to improve their use for distinct exosome subpopulations in clinical practices are also discussed. Furthermore, an overview of the most commonly encountered technical and biological challenges for effective separation of exosome subpopulations is presented.en_US
dc.description.peerreviewedYesen_US
dc.languageengen_US
dc.publisherRoyal Society of Chemistry (RSC)en_US
dc.relation.ispartofjournalAnalysten_US
dc.subject.fieldofresearchAnalytical Chemistryen_US
dc.subject.fieldofresearchOther Chemical Sciencesen_US
dc.subject.fieldofresearchcode0301en_US
dc.subject.fieldofresearchcode0399en_US
dc.titleSeparation of distinct exosome subpopulations: isolation and characterization approaches and their associated challengesen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Articlesen_US
dcterms.bibliographicCitationSingh, K; Nalabotala, R; Koo, KM; Bose, S; Nayak, R; Shiddiky, MJA, Separation of distinct exosome subpopulations: isolation and characterization approaches and their associated challenges, Analyst, 2021en_US
dc.date.updated2021-05-23T23:54:07Z
dc.description.versionAccepted Manuscript (AM)en_US
gro.description.notepublicThis publication has been entered in Griffith Research Online as an advanced online version.en_US
gro.rights.copyright© 2021 Royal Society of Chemistry. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal website for access to the definitive, published version.en_US
gro.hasfulltextFull Text
gro.griffith.authorShiddiky, Muhammad J.


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