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dc.contributor.authorChikowe, Getmore
dc.contributor.authorMpala, Lindiwe
dc.contributor.authorCock, Ian Edwin
dc.date.accessioned2021-06-02T00:07:28Z
dc.date.available2021-06-02T00:07:28Z
dc.date.issued2019
dc.identifier.issn2249-0159
dc.identifier.doi10.5530/pc.2019.2.10
dc.identifier.urihttp://hdl.handle.net/10072/404812
dc.description.abstractIntroduction: The development of multi-antibiotic resistant strains of bacteria has necessitated the search for new effective antibacterial therapies. Many Callistemon spp. were used traditionally to treat pathogenic illness and are rich in terpenoids with reported antibacterial activity. Despite this, the antibacterial activity of C. linearis leaf extracts has not been extensively examined. Methods: The ability of C. linearis leaf extracts to inhibit the growth of gram-negative and gram-positive bacterial species was investigated by disc diffusion and growth time course assays. The growth inhibitory activity was further quantified by MIC determination. Toxicity was determined using the Artemia franciscana nauplii bioassay. Results: The methanolic and aqueous C. linearis leaf extracts were good inhibitors of the growth of gram-positive bacteria yet were completely ineffective against gram-negative bacteria. The methanolic extract was a particularly good inhibitor of B. cereus and S. pyogenes growth, with MIC values of 610 and 354μg/mL respectively. The aqueous extract was also a good inhibitor of these bacteria (MICs of 927 and 660μg/mL respectively). Whilst the extracts also inhibited the growth of S. aureus and S. epidermidis, the MIC values (in the range 1200-1500μg/mL) were indicative of moderate inhibitory activity. The methanolic extracts were further investigated by growth time course assays that showed significant growth inhibition within 1h of exposure. All extracts were determined to be nontoxic in the Artemia franciscana nauplii bioassay, indicating their safety for the treatment of gram-positive bacterial infections. Conclusion: The lack of toxicity of the C. linearis leaf extracts and their growth inhibitory bioactivity against the gram-positive bacteria indicate their potential in the development of new antibiotic chemotherapies
dc.description.peerreviewedYes
dc.publisherPharmacognosy Network Worldwide
dc.relation.ispartofpagefrom39
dc.relation.ispartofpageto46
dc.relation.ispartofissue2
dc.relation.ispartofjournalPharmacognosy Communications
dc.relation.ispartofvolume9
dc.subject.fieldofresearchPharmacogenomics
dc.subject.fieldofresearchPlant Biology
dc.subject.fieldofresearchPharmacology and Pharmaceutical Sciences
dc.subject.fieldofresearchcode111505
dc.subject.fieldofresearchcode0607
dc.subject.fieldofresearchcode1115
dc.titleCallistemon linearis Schrad. and J.C. Wendl. Extracts Inhibit the Growth of Gram-Positive Bacteria but have no Effect on Gram Negative Bacteria
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationChikowe, G; Mpala, L; Cock, IE, Callistemon linearis Schrad. and J.C. Wendl. Extracts Inhibit the Growth of Gram-Positive Bacteria but have no Effect on Gram Negative Bacteria, Pharmacognosy Communications, 2019, 9 (2), pp. 39-46
dcterms.licensehttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.date.updated2021-06-01T21:48:58Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© The Author(s) 2019. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International (CC BY-NC-ND 4.0) License (http://creativecommons.org/licenses/by-nc-nd/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorCock, Ian E.
gro.griffith.authorChikowe, Getmore R.
gro.griffith.authorMpala, Lindiwe N.


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