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  • Fluorescence characteristics of E. faecalis in dentine following treatment with oxidizing endodontic irrigants

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    Version of Record (VoR)
    Author(s)
    Sin, Jonathan H
    Ipe, Deepak S
    Hamlet, Stephen
    Walsh, Laurence J
    Love, Robert M
    George, Roy
    Griffith University Author(s)
    George, Roy
    Love, Robert M.
    Hamlet, Stephen
    Year published
    2021
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    Abstract
    INTRODUCTION: This study aimed to assess changes in the fluorescence characteristics of Enterococcus faecalis in human dentine over a period of 24 hours following treatment with endodontic irrigants. METHOD: Sterilised, non-functional extracted third molars were embedded in acrylic resin and uniformly sectioned into 2mm thick dentine sections. After the removal of smear layer, the dentine sections were inoculated with E. faecalis and cultured for 7 days. The infected dentine sections were subsequently treated with different concentrations of sodium hypochlorite (NaOCl) and hydrogen peroxide (H2O2). Bacterial fluorescence ...
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    INTRODUCTION: This study aimed to assess changes in the fluorescence characteristics of Enterococcus faecalis in human dentine over a period of 24 hours following treatment with endodontic irrigants. METHOD: Sterilised, non-functional extracted third molars were embedded in acrylic resin and uniformly sectioned into 2mm thick dentine sections. After the removal of smear layer, the dentine sections were inoculated with E. faecalis and cultured for 7 days. The infected dentine sections were subsequently treated with different concentrations of sodium hypochlorite (NaOCl) and hydrogen peroxide (H2O2). Bacterial fluorescence readings were assessed at different time points using a calibrated laser device. All data were assessed for normality (Kolmogorov Smirnoff test) and analysed using ANOVA with Bonferroni post-hoc tests. RESULTS: Fluorescence readings were quenched when E. faecalis infected human dentine sections were treated with oxidizing irrigants in vitro. Throughout a 24-hour period, fluorescence recovered in part but did not return to baseline level. CONCLUSION: The fluorescence quenching effect of these oxidizing agents needs to be considered when using laser fluorescence in assessing the quality of root canal debridement or disinfection.
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    Journal Title
    Photodiagnosis and Photodynamic Therapy
    DOI
    https://doi.org/10.1016/j.pdpdt.2021.102344
    Copyright Statement
    © 2021 Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence (http://creativecommons.org/licenses/by-nc-nd/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited.
    Note
    This publication has been entered in Griffith Research Online as an advanced online version.
    Subject
    Dentistry
    Oncology and carcinogenesis
    Bacterial detection
    Dentistry
    Endodontic irrigants
    Endodontics
    Enterococcus faecalis
    Publication URI
    http://hdl.handle.net/10072/404909
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    • Journal articles

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