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  • Light-Emitting Diode Excitation for Upconversion Microscopy: A Quantitative Assessment

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    Packer493905-Accepted.pdf (793.8Kb)
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    Accepted Manuscript (AM)
    Author(s)
    Cao, Yueying
    Zheng, Xianlin
    De Camillis, Simone
    Shi, Bingyang
    Piper, James A
    Packer, Nicolle H
    Lu, Yiqing
    Griffith University Author(s)
    Packer, Nicki
    Year published
    2020
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    Abstract
    Lanthanide-based upconversion nanoparticles (UCNPs) generally require high power laser excitation. Here, we report wide-field upconversion microscopy at single-nanoparticle sensitivity using incoherent excitation of a 970 nm light-emitting diode (LED). We show that due to its broad emission spectrum, LED excitation is about 3 times less effective for UCNPs and generates high background compared to laser illumination. To counter this, we use time-gated luminescence detection to eliminate the residual background from the LED source, so that individual UCNPs with high sensitizer (Yb3+) doping and inert shell protection become ...
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    Lanthanide-based upconversion nanoparticles (UCNPs) generally require high power laser excitation. Here, we report wide-field upconversion microscopy at single-nanoparticle sensitivity using incoherent excitation of a 970 nm light-emitting diode (LED). We show that due to its broad emission spectrum, LED excitation is about 3 times less effective for UCNPs and generates high background compared to laser illumination. To counter this, we use time-gated luminescence detection to eliminate the residual background from the LED source, so that individual UCNPs with high sensitizer (Yb3+) doping and inert shell protection become clearly identified under LED excitation at 1.18 W cm–2, as confirmed by correlated electron microscopy images. Hydrophilic UCNPs are obtained by polysaccharide coating via a facile ligand exchange protocol to demonstrate imaging of cellular uptake using LED excitation. These results suggest a viable approach to bypassing the limitations associated with high-power lasers when applying UCNPs and upconversion microscopy to life science research.
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    Journal Title
    Nano Letters
    Volume
    20
    Issue
    12
    DOI
    https://doi.org/10.1021/acs.nanolett.0c02697
    Copyright Statement
    This document is the Accepted Manuscript version of a Published Work that appeared in final form in Nano Letters, copyright 2020 American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.nanolett.0c02697
    Subject
    Nanotechnology
    Nanoelectronics
    Science & Technology
    Physical Sciences
    Chemistry, Multidisciplinary
    Chemistry, Physical
    Publication URI
    http://hdl.handle.net/10072/404947
    Collection
    • Journal articles

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