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dc.contributor.authorCao, Yueying
dc.contributor.authorZheng, Xianlin
dc.contributor.authorDe Camillis, Simone
dc.contributor.authorShi, Bingyang
dc.contributor.authorPiper, James A
dc.contributor.authorPacker, Nicolle H
dc.contributor.authorLu, Yiqing
dc.date.accessioned2021-06-04T06:05:53Z
dc.date.available2021-06-04T06:05:53Z
dc.date.issued2020
dc.identifier.issn1530-6984
dc.identifier.doi10.1021/acs.nanolett.0c02697
dc.identifier.urihttp://hdl.handle.net/10072/404947
dc.description.abstractLanthanide-based upconversion nanoparticles (UCNPs) generally require high power laser excitation. Here, we report wide-field upconversion microscopy at single-nanoparticle sensitivity using incoherent excitation of a 970 nm light-emitting diode (LED). We show that due to its broad emission spectrum, LED excitation is about 3 times less effective for UCNPs and generates high background compared to laser illumination. To counter this, we use time-gated luminescence detection to eliminate the residual background from the LED source, so that individual UCNPs with high sensitizer (Yb3+) doping and inert shell protection become clearly identified under LED excitation at 1.18 W cm–2, as confirmed by correlated electron microscopy images. Hydrophilic UCNPs are obtained by polysaccharide coating via a facile ligand exchange protocol to demonstrate imaging of cellular uptake using LED excitation. These results suggest a viable approach to bypassing the limitations associated with high-power lasers when applying UCNPs and upconversion microscopy to life science research.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherAmerican Chemical Society (ACS Publications)
dc.relation.ispartofpagefrom8487
dc.relation.ispartofpageto8492
dc.relation.ispartofissue12
dc.relation.ispartofjournalNano Letters
dc.relation.ispartofvolume20
dc.subject.fieldofresearchNanotechnology
dc.subject.fieldofresearchNanoelectronics
dc.subject.fieldofresearchcode4018
dc.subject.fieldofresearchcode401804
dc.subject.keywordsScience & Technology
dc.subject.keywordsPhysical Sciences
dc.subject.keywordsChemistry, Multidisciplinary
dc.subject.keywordsChemistry, Physical
dc.titleLight-Emitting Diode Excitation for Upconversion Microscopy: A Quantitative Assessment
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationCao, Y; Zheng, X; De Camillis, S; Shi, B; Piper, JA; Packer, NH; Lu, Y, Light-Emitting Diode Excitation for Upconversion Microscopy: A Quantitative Assessment, Nano Letters, 2020, 20 (12), pp. 8487-8492
dc.date.updated2021-06-04T06:03:14Z
dc.description.versionAccepted Manuscript (AM)
gro.rights.copyrightThis document is the Accepted Manuscript version of a Published Work that appeared in final form in Nano Letters, copyright 2020 American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.nanolett.0c02697
gro.hasfulltextFull Text
gro.griffith.authorPacker, Nicki


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