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dc.contributor.authorGamage, Hasinika KAH
dc.contributor.authorChong, Raymond WW
dc.contributor.authorBucio-Noble, Daniel
dc.contributor.authorKautto, Liisa
dc.contributor.authorHardikar, Anandwardhan A
dc.contributor.authorBall, Malcolm S
dc.contributor.authorMolloy, Mark P
dc.contributor.authorPacker, Nicolle H
dc.contributor.authorPaulsen, Ian T
dc.date.accessioned2021-06-04T06:12:00Z
dc.date.available2021-06-04T06:12:00Z
dc.date.issued2020
dc.identifier.issn1949-0976
dc.identifier.doi10.1080/19490976.2020.1802209
dc.identifier.urihttp://hdl.handle.net/10072/404948
dc.description.abstractThe colonic mucus layer, comprised of highly O-glycosylated mucins, is vital to mediating host-gut microbiota interactions, yet the impact of dietary changes on colonic mucin O-glycosylation and its associations with the gut microbiota remains unexplored. Here, we used an array of omics techniques including glycomics to examine the effect of dietary fiber consumption on the gut microbiota, colonic mucin O-glycosylation and host physiology of high-fat diet-fed C57BL/6J mice. The high-fat diet group had significantly impaired glucose tolerance and altered liver proteome, gut microbiota composition, and short-chain fatty acid production compared to normal chow diet group. While dietary fiber inclusion did not reverse all high fat-induced modifications, it resulted in specific changes, including an increase in the relative abundance of bacterial families with known fiber digesters and a higher propionate concentration. Conversely, colonic mucin O-glycosylation remained similar between the normal chow and high-fat diet groups, while dietary fiber intervention resulted in major alterations in O-glycosylation. Correlation network analysis revealed previously undescribed associations between specific bacteria and mucin glycan structures. For example, the relative abundance of the bacterium Parabacteroides distasonis positively correlated with glycan structures containing one terminal fucose and correlated negatively with glycans containing two terminal fucose residues or with both an N-acetylneuraminic acid and a sulfate residue. This is the first comprehensive report of the impact of dietary fiber on the colonic mucin O-glycosylation and associations of these mucosal glycans with specific gut bacteria.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherTaylor & Francis Inc
dc.relation.ispartofpagefrome1802209
dc.relation.ispartofissue1
dc.relation.ispartofjournalGut Microbes
dc.relation.ispartofvolume12
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchcode0605
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsGastroenterology & Hepatology
dc.subject.keywordsMicrobiology
dc.subject.keywordsgut microbiota
dc.titleChanges in dietary fiber intake in mice reveal associations between colonic mucinO-glycosylation and specific gut bacteria
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationGamage, HKAH; Chong, RWW; Bucio-Noble, D; Kautto, L; Hardikar, AA; Ball, MS; Molloy, MP; Packer, NH; Paulsen, IT, Changes in dietary fiber intake in mice reveal associations between colonic mucinO-glycosylation and specific gut bacteria, Gut Microbes, 2020, 12 (1), pp. e1802209
dcterms.licensehttps://creativecommons.org/licenses/by/4.0/
dc.date.updated2021-06-04T06:09:11Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2020 The Author(s). Published with license by Taylor & Francis Group, LLC. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorPacker, Nicki


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