• myGriffith
    • Staff portal
    • Contact Us⌄
      • Future student enquiries 1800 677 728
      • Current student enquiries 1800 154 055
      • International enquiries +61 7 3735 6425
      • General enquiries 07 3735 7111
      • Online enquiries
      • Staff phonebook
    View Item 
    •   Home
    • Griffith Research Online
    • Journal articles
    • View Item
    • Home
    • Griffith Research Online
    • Journal articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

  • All of Griffith Research Online
    • Communities & Collections
    • Authors
    • By Issue Date
    • Titles
  • This Collection
    • Authors
    • By Issue Date
    • Titles
  • Statistics

  • Most Popular Items
  • Statistics by Country
  • Most Popular Authors
  • Support

  • Contact us
  • FAQs
  • Admin login

  • Login
  • Naked eye evaluation and quantitative detection of the sugarcane leaf scald pathogen, Xanthomonas albilineans, in sugarcane xylem sap

    View/Open
    Shiddiky496005-Accepted.pdf (1.559Mb)
    File version
    Accepted Manuscript (AM)
    Author(s)
    Umer, Muhammad
    Aziz, Nahian Binte
    Al Jabri, Salma
    Bhuiyan, Shamsul A
    Shiddiky, Muhammad JA
    Griffith University Author(s)
    Umer, Muhammad
    Shiddiky, Muhammad J.
    Bhuiyan, Shamsul
    Aziz, Nahian Binte
    Year published
    2021
    Metadata
    Show full item record
    Abstract
    Sugarcane leaf scald caused by the bacterium Xanthomonas albilineans is a major disease of sugarcane worldwide. Whereas erratic symptoms make phenotypic detection challenging, molecular methods require expensive instruments and labour, and longer sample-to-answer times. We report a novel method for detection of X. albilineans DNA in sugarcane xylem sap. The method involves (i) boiling lysis-based DNA extraction from sugarcane sap; (ii) magnetic purification of target sequences directly from the lysate through use of magnetic bead-bound capture probes; and (iii) DNA sandwich hybridisation platform for HRP/TMB/H2O2 reaction-based ...
    View more >
    Sugarcane leaf scald caused by the bacterium Xanthomonas albilineans is a major disease of sugarcane worldwide. Whereas erratic symptoms make phenotypic detection challenging, molecular methods require expensive instruments and labour, and longer sample-to-answer times. We report a novel method for detection of X. albilineans DNA in sugarcane xylem sap. The method involves (i) boiling lysis-based DNA extraction from sugarcane sap; (ii) magnetic purification of target sequences directly from the lysate through use of magnetic bead-bound capture probes; and (iii) DNA sandwich hybridisation platform for HRP/TMB/H2O2 reaction-based naked eye visualisation and electrochemical detection of the target. The method is sensitive (limit of detection 100 fM) and reproducible (relative standard deviation <7%) with linear dynamic range 100 fM-1 nM (R2 = 0.99). The method was tested on a range of sugarcane cultivars of known resistance ratings (susceptible, intermediate resistant, and resistant) for leaf scald disease from an inoculated field trial. Detection levels agreed with the resistance rating of cultivars tested. In addition, qPCR results strongly correlated with our assay (r = 0.91-0.99, P < 0.01) and cultivar resistance rating. We believe that our assay could be useful for rapid screening as well as sensitive quantification of target pathogen DNA in infected sugarcane plants.
    View less >
    Journal Title
    Crop & Pasture Science
    Volume
    72
    Issue
    5
    DOI
    https://doi.org/10.1071/CP20416
    Copyright Statement
    © 2021 CSIRO. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
    Subject
    Environmental sciences
    Agricultural, veterinary and food sciences
    Science & Technology
    Life Sciences & Biomedicine
    Agriculture, Multidisciplinary
    Agriculture
    leaf scald disease
    Publication URI
    http://hdl.handle.net/10072/405117
    Collection
    • Journal articles

    Footer

    Disclaimer

    • Privacy policy
    • Copyright matters
    • CRICOS Provider - 00233E

    Tagline

    • Gold Coast
    • Logan
    • Brisbane - Queensland, Australia
    First Peoples of Australia
    • Aboriginal
    • Torres Strait Islander