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dc.contributor.authorA. Da⿿Dara, Akramen_US
dc.contributor.authorS. Li, Yueshengen_US
dc.contributor.authorXiong, Tieen_US
dc.contributor.authorZhou, Jieen_US
dc.contributor.authorWilliams, Gail M.en_US
dc.contributor.authorP. McManus, Donalden_US
dc.contributor.authorFeng, Zhengen_US
dc.contributor.authorL. Yu, Xinen_US
dc.contributor.authorJ. Gray, Darrenen_US
dc.contributor.authorA. Harn, Donalden_US
dc.date.accessioned2017-05-03T14:01:23Z
dc.date.available2017-05-03T14:01:23Z
dc.date.issued2008en_US
dc.date.modified2011-08-30T06:22:57Z
dc.identifier.issn1873-2518en_US
dc.identifier.doi10.1016/j.vaccine.2008.04.080en_AU
dc.identifier.urihttp://hdl.handle.net/10072/40516
dc.description.abstractSchistosomiasis japonica is an endemic, zoonotic disease of major public health importance in China where water buffaloes account for approximately 75% of disease transmission. Interventions that reduce schistosome infection in water buffaloes will enhance their health simultaneously reducing disease transmission to humans. While chemotherapy has proved successful, it requires continued time consuming and expensive mass treatments. A more sustainable option would be development of vaccines that reduce transmission of S. japonicum from bovines to replace bovine chemotherapy. We performed two randomized double blind trials in water buffaloes to determine if DNA vaccines encoding triose-phosphate isomerase (SjCTPI), or the tetraspanin 23 kDa integral membrane protein (SjC23), alone or fused to bovine heat shock protein 70 (Hsp70) could induce a level of immunity conducive to long-term sustainable control. Groups of water buffaloes (15/group) received three intramuscular injections, 4 weeks apart. Booster immunizations were co-administered with a plasmid DNA encoding IL-12. Four weeks after the last injection, water buffaloes were challenged with 1000 cercariae, and vaccine efficacy analyzed 8 weeks later. Water buffaloes vaccinated with SjCTPI-Hsp70 or SjCTPI plasmids had worm burdens reduced by 51.2% and 41.5%, respectively. Importantly, fecal miracidial hatching was reduced by 52.1% and 33.2% respectively compared to control vaccinated water buffaloes. Vaccination with SjC23-Hsp70 and SjC23 plasmids reduced worm burdens by 50.9% and 45.5%, respectively, and fecal miracidial hatching by 52.0% and 47.4%. A mathematical model of schistosome transmission predicts that schistosome vaccines capable of reducing water buffaloes' fecal egg output by 45%, alone or in conjunction with praziquantel treatment, will lead to a significant reduction in transmission of schistosomiasis. Both DNA vaccines tested here exceed this hypothetical level. Indeed, mathematical modeling of SjCTPI-Hsp70 and SjC23-Hsp70 alone and in conjunction with human chemotherapy showed a significant reduction in transmission almost to the point of elimination.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_AU
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherElsevieren_US
dc.publisher.placeUnited Kingdomen_US
dc.relation.ispartofstudentpublicationYen_AU
dc.relation.ispartofpagefrom3617en_US
dc.relation.ispartofpageto3625en_US
dc.relation.ispartofissue29-30en_US
dc.relation.ispartofjournalVaccineen_US
dc.relation.ispartofvolume26en_US
dc.rights.retentionYen_AU
dc.subject.fieldofresearchMedical Parasitologyen_US
dc.subject.fieldofresearchcode110803en_US
dc.titleDNA-based vaccine protects against zoonotic schistosomiasis in water buffaloen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.date.issued2008
gro.hasfulltextNo Full Text


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