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dc.contributor.authorVezina, Ben
dc.contributor.authorPetit, Guillaume A
dc.contributor.authorMartin, Jennifer L
dc.contributor.authorHalili, Maria A
dc.date.accessioned2021-06-23T04:58:11Z
dc.date.available2021-06-23T04:58:11Z
dc.date.issued2020
dc.identifier.issn1932-6203
dc.identifier.doi10.1371/journal.pone.0241306
dc.identifier.urihttp://hdl.handle.net/10072/405288
dc.description.abstractIdentification of bacterial virulence factors is critical for understanding disease pathogenesis, drug discovery and vaccine development. In this study we used two approaches to predict virulence factors of Burkholderia pseudomallei, the Gram-negative bacterium that causes melioidosis. B. pseudomallei is naturally antibiotic resistant and there are no clinically available melioidosis vaccines. To identify B. pseudomallei protein targets for drug discovery and vaccine development, we chose to search for substrates of the B. pseudomallei periplasmic disulfide bond forming protein A (DsbA). DsbA introduces disulfide bonds into extra-cytoplasmic proteins and is essential for virulence in many Gram-negative organism, including B. pseudomallei. The first approach to identify B. pseudomallei DsbA virulence factor substrates was a large-scale genomic analysis of 511 unique B. pseudomallei disease-associated strains. This yielded 4,496 core gene products, of which we hypothesise 263 are DsbA substrates. Manual curation and database screening of the 263 mature proteins yielded 81 associated with disease pathogenesis or virulence. These were screened for structural homologues to predict potential B-cell epitopes. In the second approach, we searched the B. pseudomallei genome for homologues of the more than 90 known DsbA substrates in other bacteria. Using this approach, we identified 15 putative B. pseudomallei DsbA virulence factor substrates, with two of these previously identified in the genomic approach, bringing the total number of putative DsbA virulence factor substrates to 94. The two putative B. pseudomallei virulence factors identified by both methods are homologues of PenI family β-lactamase and a molecular chaperone. These two proteins could serve as high priority targets for future B. pseudomallei virulence factor characterization.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherPublic Library Science
dc.relation.ispartofpagefrome0241306
dc.relation.ispartofissue11
dc.relation.ispartofjournalPLoS One
dc.relation.ispartofvolume15
dc.subject.fieldofresearchBiological sciences
dc.subject.fieldofresearchcode31
dc.subject.keywordsScience & Technology
dc.subject.keywordsMultidisciplinary Sciences
dc.subject.keywordsScience & Technology - Other Topics
dc.subject.keywordsDISULFIDE BOND FORMATION
dc.subject.keywordsESCHERICHIA-COLI
dc.titlePrediction of Burkholderia pseudomallei DsbA substrates identifies potential virulence factors and vaccine targets
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationVezina, B; Petit, GA; Martin, JL; Halili, MA, Prediction of Burkholderia pseudomallei DsbA substrates identifies potential virulence factors and vaccine targets, PLoS One, 2020, 15 (11), pp. e0241306
dcterms.dateAccepted2020-10-12
dcterms.licensehttps://creativecommons.org/licenses/by/4.0/
dc.date.updated2021-06-23T04:29:45Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2020 Vezina et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
gro.hasfulltextFull Text
gro.griffith.authorMartin, Jennifer


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