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dc.contributor.authorJiang, Lan
dc.contributor.authorZhu, Guoliang
dc.contributor.authorHan, Jianying
dc.contributor.authorHou, Chengjian
dc.contributor.authorZhang, Xue
dc.contributor.authorWang, Zhixin
dc.contributor.authorYuan, Weize
dc.contributor.authorLv, Kangjie
dc.contributor.authorCong, Zhanren
dc.contributor.authorWang, Xinye
dc.contributor.authorChen, Xiangyin
dc.contributor.authorKarthik, Loganathan
dc.contributor.authorYang, Huanting
dc.contributor.authorQuinn, Ronald J
dc.contributor.authoret al.
dc.description.abstractFungal terpenoids catalyzed by bifunctional terpene synthases (BFTSs) possess interesting bioactive and chemical properties. In this study, an integrated approach of genome mining, heterologous expression, and in vitro enzymatic activity assay was used, and these identified a unique BFTS sub-clade critical to the formation of a 5-15 trans-fused bicyclic sesterterpene preterpestacin I (1). The 5-15 bicyclic BFTS gene clusters were highly conserved but showed relatively wide phylogenetic distribution across several species of the diverged fungal classes Dothideomycetes and Sordariomycetes. Further genomic organization analysis of these homologous biosynthetic gene clusters from this clade revealed a glycosyltransferase from the graminaceous pathogen Bipolaris sorokiniana isolate BS11134, which was absent in other 5-15 bicyclic BFTS gene clusters. Targeted isolation guided by BFTS gene deletion led to the identification of two new sesterterpenoids (4, and 6) from BS11134. Compounds 2 and 4 showed moderate effects on LPS-induced nitrous oxide production in the murine macrophage-like cell line RAW264.7 with in vitro inhibition rates of 36.6 ± 2.4% and 24.9 ± 2.1% at 10 μM, respectively. The plausible biosynthetic pathway of these identified compounds was proposed as well. This work revealed that phytopathogenic fungi can serve as important sources of active terpenoids via systematic analysis of the genomic organization of BFTS biosynthetic gene clusters, their phylogenetic distribution in fungi, and cyclization properties of their metabolic products. KEY POINTS: • Genome mining of the first BFTS BGC harboring a glycosyltransferase. • Gene-deletion guided isolation revealed three novel 5-15 bicyclic sesterterpenoids. • Biosynthetic pathway of isolated sesterterpenoids was proposed.
dc.publisherSpringer Science and Business Media LLC
dc.relation.ispartofjournalApplied Microbiology and Biotechnology
dc.subject.fieldofresearchMedical Microbiology
dc.subject.keywordsBifunctional terpene synthases,
dc.titleGenome-guided investigation of anti-inflammatory sesterterpenoids with 5-15 trans-fused ring system from phytopathogenic fungi
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationJiang, L; Zhu, G; Han, J; Hou, C; Zhang, X; Wang, Z; Yuan, W; Lv, K; Cong, Z; Wang, X; Chen, X; Karthik, L; Yang, H; Quinn, RJ; et al., Genome-guided investigation of anti-inflammatory sesterterpenoids with 5-15 trans-fused ring system from phytopathogenic fungi, Applied Microbiology and Biotechnology, 2021
gro.description.notepublicThis publication has been entered as an advanced online version in Griffith Research Online.
gro.hasfulltextNo Full Text
gro.griffith.authorQuinn, Ronald J.
gro.griffith.authorHan, Jianying

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