dc.contributor.author | Battle, Andrew R | |
dc.contributor.author | Petrov, Evgeny | |
dc.contributor.author | Pal, Prithwish | |
dc.contributor.author | Martinac, Boris | |
dc.date.accessioned | 2017-05-03T16:04:29Z | |
dc.date.available | 2017-05-03T16:04:29Z | |
dc.date.issued | 2009 | |
dc.date.modified | 2011-08-31T07:19:30Z | |
dc.identifier.issn | 0014-5793 | |
dc.identifier.doi | 10.1016/j.febslet.2008.12.033 | |
dc.identifier.uri | http://hdl.handle.net/10072/40554 | |
dc.description.abstract | The bacterial mechanosensitive (MS) channels of small (MscS) and large (MscL) conductance have functionally been reconstituted into giant unilamellar liposomes (GUVs) using an improved reconstitution method in the presence of sucrose. This method gives significant time savings (preparation times as little as 6 h) compared to the classical method of protein reconstitution which uses a dehydration/rehydration (D/R) procedure (minimum 2 days preparation time). Moreover, it represents the first highly reproducible method for functional reconstitution of MscS as well as MscS/MscL co-reconstitution. This novel procedure has the potential to be used for studies of other ion channels by liposome reconstitution. | |
dc.description.peerreviewed | Yes | |
dc.description.publicationstatus | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Elsevier | |
dc.publisher.place | Netherlands | |
dc.relation.ispartofstudentpublication | N | |
dc.relation.ispartofpagefrom | 407 | |
dc.relation.ispartofpageto | 412 | |
dc.relation.ispartofissue | 2 | |
dc.relation.ispartofjournal | FEBS Letters | |
dc.relation.ispartofvolume | 583 | |
dc.rights.retention | Y | |
dc.subject.fieldofresearch | Medicinal and biomolecular chemistry | |
dc.subject.fieldofresearch | Biochemistry and cell biology | |
dc.subject.fieldofresearch | Biochemistry and cell biology not elsewhere classified | |
dc.subject.fieldofresearch | Evolutionary biology | |
dc.subject.fieldofresearchcode | 3404 | |
dc.subject.fieldofresearchcode | 3101 | |
dc.subject.fieldofresearchcode | 310199 | |
dc.subject.fieldofresearchcode | 3104 | |
dc.title | Rapid and improved reconstitution of bacterial mechanosensitive ion channel proteins MscS and MscL into liposomes using a modified sucrose method | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
gro.date.issued | 2009 | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Battle, Andrew | |