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dc.contributor.authorSoda, Narshone
dc.contributor.authorGonzaga, Zennia Jean
dc.contributor.authorChen, Shuxiong
dc.contributor.authorKoo, Kevin M
dc.contributor.authorNguyen, Nam-Trung
dc.contributor.authorShiddiky, Muhammad JA
dc.contributor.authorRehm, Bernd HA
dc.date.accessioned2021-07-05T04:23:10Z
dc.date.available2021-07-05T04:23:10Z
dc.date.issued2021
dc.identifier.issn1944-8244
dc.identifier.doi10.1021/acsami.1c05355
dc.identifier.urihttp://hdl.handle.net/10072/405668
dc.description.abstractEarly sensitive diagnosis of cancer is critical for enhancing treatment success. We previously bioengineered multifunctional core-shell structures composed of a poly-3-hydroxybutyrate (PHB) core densely coated with protein functions for uses in bioseparation and immunodiagnostic applications. Here, we report bioengineering of Escherichia coli to self-assemble PHB inclusions that codisplay a ferritin-derived iron-binding peptide and the protein A-derived antibody-binding Z domain. The iron-binding peptide mediated surface coating with a ferrofluid imparting superparamagnetic properties, while the Z domain remained accessible for binding of cancer biomarker-specific antibodies. We demonstrated that these nanobeads can specifically bind biomarkers in complex mixtures, enabling efficient magnetic separation toward enhanced electrochemical detection of cancer biomarkers such as methylated DNA and exosomes from cancer cells. Our study revealed that superparamagnetic core-shell structures can be derived from biological self-assembly systems for uses in sensitive and specific electrochemical detection of cancer biomarkers, laying the foundation for engineering advanced nanomaterials for diverse diagnostic approaches.
dc.description.peerreviewedYes
dc.languageeng
dc.publisherAmerican Chemical Society (ACS)
dc.relation.ispartofjournalACS Appl Mater Interfaces
dc.subject.fieldofresearchChemical Sciences
dc.subject.fieldofresearchEngineering
dc.subject.fieldofresearchcode03
dc.subject.fieldofresearchcode09
dc.subject.keywordsDNA methylation
dc.subject.keywordselectrochemical biosensor
dc.subject.keywordsexosomes
dc.subject.keywordsnanobeads
dc.subject.keywordspoly-3-hydroxybutyrate
dc.titleBioengineered Polymer Nanobeads for Isolation and Electrochemical Detection of Cancer Biomarkers
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationSoda, N; Gonzaga, ZJ; Chen, S; Koo, KM; Nguyen, N-T; Shiddiky, MJA; Rehm, BHA, Bioengineered Polymer Nanobeads for Isolation and Electrochemical Detection of Cancer Biomarkers, ACS Appl Mater Interfaces, 2021
dc.date.updated2021-07-01T04:09:56Z
gro.description.notepublicThis publication has been entered in Griffith Research Online as an advanced online version.
gro.hasfulltextNo Full Text
gro.griffith.authorSoda, Narshone
gro.griffith.authorShiddiky, Muhammad J.
gro.griffith.authorRehm, Bernd
gro.griffith.authorGonzaga, Jean C.
gro.griffith.authorChen, Shuxiong
gro.griffith.authorNguyen, Nam-Trung


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