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dc.contributor.authorDesai, Devika
dc.contributor.authorGoh, Kelvin GK
dc.contributor.authorSullivan, Matthew J
dc.contributor.authorChattopadhyay, Debasish
dc.contributor.authorUlett, Glen C
dc.date.accessioned2021-07-15T00:29:50Z
dc.date.available2021-07-15T00:29:50Z
dc.date.issued2021
dc.identifier.issn1438-4221
dc.identifier.doi10.1016/j.ijmm.2021.151520
dc.identifier.urihttp://hdl.handle.net/10072/405961
dc.description.abstractStreptococcus agalactiae, also known as group B Streptococcus, is an aetiological agent of urinary tract infection (UTI) in adults, including cystitis, pyelonephritis and asymptomatic bacteriuria (ABU). Whereas ABU-causing S. agalactiae (ABSA) have been shown to grow and achieve higher culture denstity in human urine compared to uropathogenic S. agalactiae (UPSA) other phenotypic distinctions between S. agalactiae isolated from different forms of UTI are not known. Here, we define the hemolytic activities and biofilm-formation of a collection of clinical isolates of UPSA, ABSA and recurrent S. agalactiae bacteriuria (rSAB) strains to explore these phenotypes in the context of clinical history of isolates. A total of 61 UPSA, 184 ABSA, and 47 rSAB isolates were analyzed for relative hemolytic activity by spot assay on blood agar, which was validated using a erythrocyte lysis suspension assay. Biofilm formation was determined by microtiter plate assay with Lysogeny and Todd-Hewitt broths supplemented with 1% glucose to induce biofilm formation. We also used multiplex PCR to analyze isolates for the presence of genes encoding adhesive pili, which contribute to biofilm formation. Comparing the hemolytic activities of 292 isolates showed, surprisingly, that ABSA strains were significantly more likely to be highly hemolytic compared to other strains. In contrast, there were no differences between the relative abilities of strains from the different clinical history groups to form biofilms. Taken together, these findings demonstrate a propensity of S. agalactiae causing ABU to be highly hemolytic but no link between clinical history of UTI strains and ability to form biofilm.
dc.description.peerreviewedYes
dc.languageen
dc.publisherElsevier BV
dc.relation.ispartofjournalInternational Journal of Medical Microbiology
dc.subject.fieldofresearchMedical microbiology
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchcode3207
dc.subject.fieldofresearchcode3107
dc.titleHemolytic activity and biofilm-formation among clinical isolates of group B streptococcus causing acute urinary tract infection and asymptomatic bacteriuria
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationDesai, D; Goh, KGK; Sullivan, MJ; Chattopadhyay, D; Ulett, GC, Hemolytic activity and biofilm-formation among clinical isolates of group B streptococcus causing acute urinary tract infection and asymptomatic bacteriuria, International Journal of Medical Microbiology, 2021
dcterms.licensehttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.date.updated2021-07-12T22:55:46Z
dc.description.versionVersion of Record (VoR)
gro.description.notepublicThis publication has been entered as an advanced online version in Griffith Research Online.
gro.rights.copyright© 2021 The Authors. Published by Elsevier GmbH. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International (CC BY-NC-ND 4.0) License, which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorUlett, Glen C.


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