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dc.contributor.authorJennings, Michaelen_US
dc.contributor.authorJen, Fredaen_US
dc.contributor.authorF. Roddam, Louiseen_US
dc.contributor.authorA. Apicella, Michaelen_US
dc.contributor.authorL. Edwards, Jenniferen_US
dc.date.accessioned2017-04-24T13:14:15Z
dc.date.available2017-04-24T13:14:15Z
dc.date.issued2011en_US
dc.date.modified2011-09-05T06:47:22Z
dc.identifier.issn14625822en_US
dc.identifier.doi10.1111/j.1462-5822.2011.01586.xen_AU
dc.identifier.urihttp://hdl.handle.net/10072/40606
dc.description.abstractExpression of type IV pili by Neisseria gonorrhoeae plays a critical role in mediating adherence to human epithelial cells. Gonococcal pilin is modified with an O-linked glycan, which may be present as a di- or monosaccharide because of phase variation of select pilin glycosylation genes. It is accepted that bacterial proteins may be glycosylated; less clear is how the protein glycan may mediate virulence. Using primary, human, cervical epithelial (i.e. pex) cells, we now provide evidence to indicate that the pilin glycan mediates productive cervical infection. In this regard, pilin glycan-deficient mutant gonococci exhibited an early hyper-adhesive phenotype but were attenuated in their ability to invade pex cells. Our data further indicate that the pilin glycan was required for gonococci to bind to the I-domain region of complement receptor 3, which is naturally expressed by pex cells. Comparative, quantitative, infection assays revealed that mutant gonococci lacking the pilin glycan did not bind to the I-domain when it is in a closed, low-affinity conformation and cannot induce an active conformation to complement receptor 3 during pex cell challenge. To our knowledge, these are the first data to directly demonstrate how a protein-associated bacterial glycan may contribute to pathogenesis.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_AU
dc.languageEnglishen_US
dc.language.isoen_AU
dc.publisherWiley-Blackwell Publishing Ltd.en_US
dc.publisher.placeUnited Kingdomen_US
dc.relation.ispartofstudentpublicationNen_AU
dc.relation.ispartofpagefrom885en_US
dc.relation.ispartofpageto896en_US
dc.relation.ispartofissue6en_US
dc.relation.ispartofjournalCellular Microbiologyen_US
dc.relation.ispartofvolume13en_US
dc.rights.retentionYen_AU
dc.subject.fieldofresearchMedical Microbiology not elsewhere classifieden_US
dc.subject.fieldofresearchcode110899en_US
dc.titleNeisseria gonorrhoeae pilin glycan contributes to CR3 activation during challenge of primary cervical epithelial cellsen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.facultyOffice of the Snr Dep Vice Chancellor, Institute for Glycomicsen_US
gro.date.issued2011
gro.hasfulltextNo Full Text


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