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dc.contributor.authorJennings, Michaelen_US
dc.contributor.authorJen, Fredaen_US
dc.contributor.authorF. Roddam, Louiseen_US
dc.contributor.authorA. Apicella, Michaelen_US
dc.contributor.authorL. Edwards, Jenniferen_US
dc.description.abstractExpression of type IV pili by Neisseria gonorrhoeae plays a critical role in mediating adherence to human epithelial cells. Gonococcal pilin is modified with an O-linked glycan, which may be present as a di- or monosaccharide because of phase variation of select pilin glycosylation genes. It is accepted that bacterial proteins may be glycosylated; less clear is how the protein glycan may mediate virulence. Using primary, human, cervical epithelial (i.e. pex) cells, we now provide evidence to indicate that the pilin glycan mediates productive cervical infection. In this regard, pilin glycan-deficient mutant gonococci exhibited an early hyper-adhesive phenotype but were attenuated in their ability to invade pex cells. Our data further indicate that the pilin glycan was required for gonococci to bind to the I-domain region of complement receptor 3, which is naturally expressed by pex cells. Comparative, quantitative, infection assays revealed that mutant gonococci lacking the pilin glycan did not bind to the I-domain when it is in a closed, low-affinity conformation and cannot induce an active conformation to complement receptor 3 during pex cell challenge. To our knowledge, these are the first data to directly demonstrate how a protein-associated bacterial glycan may contribute to pathogenesis.en_US
dc.publisherWiley-Blackwell Publishing Ltd.en_US
dc.publisher.placeUnited Kingdomen_US
dc.relation.ispartofjournalCellular Microbiologyen_US
dc.subject.fieldofresearchMedical Microbiology not elsewhere classifieden_US
dc.titleNeisseria gonorrhoeae pilin glycan contributes to CR3 activation during challenge of primary cervical epithelial cellsen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.facultyOffice of the Snr Dep Vice Chancellor, Institute for Glycomicsen_US
gro.hasfulltextNo Full Text

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