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dc.contributor.authorShi, Lei
dc.contributor.authorWang, Yue
dc.contributor.authorIto, Fumiya
dc.contributor.authorOkazaki, Yasumasa
dc.contributor.authorTanaka, Hiromasa
dc.contributor.authorMizuno, Masaaki
dc.contributor.authorHori, Masaru
dc.contributor.authorRichardson, Des R
dc.contributor.authorToyokuni, Shinya
dc.date.accessioned2021-07-27T00:16:48Z
dc.date.available2021-07-27T00:16:48Z
dc.date.issued2016
dc.identifier.issn0003-9861
dc.identifier.doi10.1016/j.abb.2016.05.016
dc.identifier.urihttp://hdl.handle.net/10072/406340
dc.description.abstractNon-thermal plasma (NTP) is a recently developed technology that elicits a variety of biological effects. This includes cancer cell-specific cytotoxicity, which is mainly attributed to the regional generation of reactive oxygen species (ROS). We studied the effects of NTP on malignant mesothelioma (MM) and its modulation by L-ascorbate. L-ascorbate is a major water-soluble anti-oxidant in vivo, but its pro-oxidant activity in vitro has been well recognized. Thus, the effects of ascorbate on the efficacy of NTP is important to examine. NTP exposure dose-dependently killed MM cells, whereas MM cells tolerated 1 mM L-ascorbate. However, brief pre-treatment with a pharmacological dose (250–750 μM) of L-ascorbate immediately prior to NTP exposure significantly increased its cytotoxicity in a dose-dependent manner, which was inhibited by the iron chelator, deferoxamine. However, paradoxically, this potentiating effect of L-ascorbate was completely abolished by a prolonged 4 h pre-incubation with L-ascorbate (500 μM). MM cytotoxicity induced by NTP was associated with immediate oxidative stress evaluated by 2′,7′-dichlorodihydrofluorecein diacetate, which was followed by an increase in the expression of the autophagosome marker, LC3B-II. In conclusion, MM can be a target for NTP treatment and L-ascorbate can increase or decrease its efficacy depending on the length of the pre-incubation period.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherElsevier
dc.relation.ispartofpagefrom109
dc.relation.ispartofpageto116
dc.relation.ispartofjournalArchives of Biochemistry and Biophysics
dc.relation.ispartofvolume605
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchcode3101
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsBiochemistry & Molecular Biology
dc.subject.keywordsBiophysics
dc.subject.keywordsL-ascorbate
dc.titleBiphasic effects of L-ascorbate on the tumoricidal activity of non-thermal plasma against malignant mesothelioma cells
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationShi, L; Wang, Y; Ito, F; Okazaki, Y; Tanaka, H; Mizuno, M; Hori, M; Richardson, DR; Toyokuni, S, Biphasic effects of L-ascorbate on the tumoricidal activity of non-thermal plasma against malignant mesothelioma cells, Archives of Biochemistry and Biophysics, 2016, 605, pp. 109-116
dcterms.dateAccepted2016-05-20
dc.date.updated2021-07-27T00:13:40Z
gro.hasfulltextNo Full Text
gro.griffith.authorRichardson, Des R.


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