dc.contributor.author | Shi, Lei | |
dc.contributor.author | Wang, Yue | |
dc.contributor.author | Ito, Fumiya | |
dc.contributor.author | Okazaki, Yasumasa | |
dc.contributor.author | Tanaka, Hiromasa | |
dc.contributor.author | Mizuno, Masaaki | |
dc.contributor.author | Hori, Masaru | |
dc.contributor.author | Richardson, Des R | |
dc.contributor.author | Toyokuni, Shinya | |
dc.date.accessioned | 2021-07-27T00:16:48Z | |
dc.date.available | 2021-07-27T00:16:48Z | |
dc.date.issued | 2016 | |
dc.identifier.issn | 0003-9861 | |
dc.identifier.doi | 10.1016/j.abb.2016.05.016 | |
dc.identifier.uri | http://hdl.handle.net/10072/406340 | |
dc.description.abstract | Non-thermal plasma (NTP) is a recently developed technology that elicits a variety of biological effects. This includes cancer cell-specific cytotoxicity, which is mainly attributed to the regional generation of reactive oxygen species (ROS). We studied the effects of NTP on malignant mesothelioma (MM) and its modulation by L-ascorbate. L-ascorbate is a major water-soluble anti-oxidant in vivo, but its pro-oxidant activity in vitro has been well recognized. Thus, the effects of ascorbate on the efficacy of NTP is important to examine. NTP exposure dose-dependently killed MM cells, whereas MM cells tolerated 1 mM L-ascorbate. However, brief pre-treatment with a pharmacological dose (250–750 μM) of L-ascorbate immediately prior to NTP exposure significantly increased its cytotoxicity in a dose-dependent manner, which was inhibited by the iron chelator, deferoxamine. However, paradoxically, this potentiating effect of L-ascorbate was completely abolished by a prolonged 4 h pre-incubation with L-ascorbate (500 μM). MM cytotoxicity induced by NTP was associated with immediate oxidative stress evaluated by 2′,7′-dichlorodihydrofluorecein diacetate, which was followed by an increase in the expression of the autophagosome marker, LC3B-II. In conclusion, MM can be a target for NTP treatment and L-ascorbate can increase or decrease its efficacy depending on the length of the pre-incubation period. | |
dc.description.peerreviewed | Yes | |
dc.language | English | |
dc.publisher | Elsevier | |
dc.relation.ispartofpagefrom | 109 | |
dc.relation.ispartofpageto | 116 | |
dc.relation.ispartofjournal | Archives of Biochemistry and Biophysics | |
dc.relation.ispartofvolume | 605 | |
dc.subject.fieldofresearch | Biochemistry and cell biology | |
dc.subject.fieldofresearchcode | 3101 | |
dc.subject.keywords | Science & Technology | |
dc.subject.keywords | Life Sciences & Biomedicine | |
dc.subject.keywords | Biochemistry & Molecular Biology | |
dc.subject.keywords | Biophysics | |
dc.subject.keywords | L-ascorbate | |
dc.title | Biphasic effects of L-ascorbate on the tumoricidal activity of non-thermal plasma against malignant mesothelioma cells | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dcterms.bibliographicCitation | Shi, L; Wang, Y; Ito, F; Okazaki, Y; Tanaka, H; Mizuno, M; Hori, M; Richardson, DR; Toyokuni, S, Biphasic effects of L-ascorbate on the tumoricidal activity of non-thermal plasma against malignant mesothelioma cells, Archives of Biochemistry and Biophysics, 2016, 605, pp. 109-116 | |
dcterms.dateAccepted | 2016-05-20 | |
dc.date.updated | 2021-07-27T00:13:40Z | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Richardson, Des R. | |