Show simple item record

dc.contributor.authorDarby, Ian D
dc.contributor.authorHosseini Bai, Shahla
dc.contributor.authorWallace, Helen M
dc.contributor.authorTrueman, Stephen J
dc.date.accessioned2021-07-27T05:01:11Z
dc.date.available2021-07-27T05:01:11Z
dc.date.issued2021
dc.identifier.issn0067-1924
dc.identifier.doi10.1071/BT21047
dc.identifier.urihttp://hdl.handle.net/10072/406376
dc.description.abstractDemand for therapeutic honey is driving establishment of Leptospermum plantations. This study developed micropropagation methods for two species – Leptospermum polygalifolium Salisb. and L. scoparium J.R.Forst. & G. Forst. The study determined how shoot proliferation and adventitious rooting were influenced by the original explant position on the seedling and the concentration of benzyladenine (BA) in the proliferation medium. Hormone-free node culture was highly effective for both species. Nodal explants often formed roots in the absence of BA and developed elongated axillary shoots. Median shoot numbers of 584 and 659 were formed in 31–32 weeks from a single L. polygalifolium or L. scoparium seed, respectively. A low BA dose was effective for callogenesis and shoot proliferation of L. polygalifolium, but not L. scoparium. The median number of shoots produced from a single L. polygalifolium seed was 630 using 2.22-mM BA. This dose induced extremely high shoot numbers in some clones because explants often produced extensive callus and multiple short shoots. Shoots formed adventitious roots without indole-3-butyric acid and plantlets were acclimatised to nursery conditions. The original explant position did not influence shoot proliferation or adventitious rooting. Leptospermum polygalifolium and L. scoparium proved amenable to micropropagation, facilitating rapid establishment of nectar plantations.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherCSIRO Publishing
dc.relation.ispartofjournalAustralian Journal of Botany
dc.subject.fieldofresearchHorticultural crop growth and development
dc.subject.fieldofresearchEcology
dc.subject.fieldofresearchEvolutionary biology
dc.subject.fieldofresearchPlant biology
dc.subject.fieldofresearchcode300802
dc.subject.fieldofresearchcode3103
dc.subject.fieldofresearchcode3104
dc.subject.fieldofresearchcode3108
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsPlant Sciences
dc.subject.keywordsadventitious rooting
dc.subject.keywordsauxin
dc.titleMicropropagation of the therapeutic-honey plants Leptospermum polygalifolium and L. scoparium (Myrtaceae)
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationDarby, ID; Hosseini Bai, S; Wallace, HM; Trueman, SJ, Micropropagation of the therapeutic-honey plants Leptospermum polygalifolium and L. scoparium (Myrtaceae), Australian Journal of Botany, 2021
dcterms.licensehttps://creativecommons.org/licenses/by/4.0/
dc.date.updated2021-07-27T01:10:40Z
dc.description.versionVersion of Record (VoR)
gro.description.notepublicThis publication has been entered in Griffith Research Online as an advanced online version.
gro.rights.copyright© CSIRO 2021. This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorTrueman, Stephen J.
gro.griffith.authorHosseini-Bai, Shahla
dc.subject.socioeconomiccode260510 Ornamentals, natives, flowers and nursery plants


Files in this item

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record