Ameliorating effects of Raphanus sativus leaves on sodium arsenite-induced perturbation of blood indices in Swiss albino mice

View/ Open
Author(s)
Dilruba, Sayada
Hasibuzzaman, MM
Rahman, Mashiur
Mohanto, Nayan Chandra
Aktar, Sharmin
Rahman, Atiqur
Hossain, Md Imam
Noman, Abu Shadat Mohammod
Nikkon, Farjana
Saud, Zahangir Alam
Hossain, Khaled
Griffith University Author(s)
Year published
2017
Metadata
Show full item recordAbstract
Objective: To evaluate the ameliorating effects of Raphanus sativus leaves (RSL) against sodium arsenite (Sa)-induced adverse effects through mice experiments. Methods:Swiss albino mice were divided into four equal groups: control, Sa, RSL, RSL + Sa. Sa (10 mg/kg body weight/day), and powder form of RSL (50 mg/kg body weight/day) were provided as food supplement orallty. Blood indices were measured using commercially available kits through colorimetric methods. Results: It was observed that lactate dehydrogenase, alkaline phosphatase, alanine aminotransferase, and aspartate aminotransferase activities were significantly (P ...
View more >Objective: To evaluate the ameliorating effects of Raphanus sativus leaves (RSL) against sodium arsenite (Sa)-induced adverse effects through mice experiments. Methods:Swiss albino mice were divided into four equal groups: control, Sa, RSL, RSL + Sa. Sa (10 mg/kg body weight/day), and powder form of RSL (50 mg/kg body weight/day) were provided as food supplement orallty. Blood indices were measured using commercially available kits through colorimetric methods. Results: It was observed that lactate dehydrogenase, alkaline phosphatase, alanine aminotransferase, and aspartate aminotransferase activities were significantly (P < 0.05) higher in Sa-treated mice than those in the control group. RSL significantly reduced Sa-induced elevation of the activities of these enzymes in serum significantly (P < 0.05). Serum butyrylcholinesterase activity and high density lipoproteins cholesterol levels in Sa-treated mice were significantly (P < 0.05) lower than the control group, and the food supplementation of RSL could significantly (P < 0.05) prevent the reduction of Sa-mediated serum butyryl cholinesterase activity and high density lipoproteins cholesterol levels. RSL could also reduce the Sa-induced elevation of serum urea level significantly (P < 0.05). Conclusions: Results of this study suggest the protective or ameliorating effects of RSL on Sa-induced perturbation of blood indices are related to the hepatic, cardiovascular and kidney dysfunction. Therefore, RSL may be useful to reduce arsenic toxicity in human in the future.
View less >
View more >Objective: To evaluate the ameliorating effects of Raphanus sativus leaves (RSL) against sodium arsenite (Sa)-induced adverse effects through mice experiments. Methods:Swiss albino mice were divided into four equal groups: control, Sa, RSL, RSL + Sa. Sa (10 mg/kg body weight/day), and powder form of RSL (50 mg/kg body weight/day) were provided as food supplement orallty. Blood indices were measured using commercially available kits through colorimetric methods. Results: It was observed that lactate dehydrogenase, alkaline phosphatase, alanine aminotransferase, and aspartate aminotransferase activities were significantly (P < 0.05) higher in Sa-treated mice than those in the control group. RSL significantly reduced Sa-induced elevation of the activities of these enzymes in serum significantly (P < 0.05). Serum butyrylcholinesterase activity and high density lipoproteins cholesterol levels in Sa-treated mice were significantly (P < 0.05) lower than the control group, and the food supplementation of RSL could significantly (P < 0.05) prevent the reduction of Sa-mediated serum butyryl cholinesterase activity and high density lipoproteins cholesterol levels. RSL could also reduce the Sa-induced elevation of serum urea level significantly (P < 0.05). Conclusions: Results of this study suggest the protective or ameliorating effects of RSL on Sa-induced perturbation of blood indices are related to the hepatic, cardiovascular and kidney dysfunction. Therefore, RSL may be useful to reduce arsenic toxicity in human in the future.
View less >
Journal Title
Asian Pacific Journal of Tropical Biomedicine
Volume
7
Issue
10
Copyright Statement
© 2017 Hainan Medical University. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://
creativecommons.org/licenses/by-nc-nd/4.0/).
Subject
Biomedical and clinical sciences
Science & Technology
Life Sciences & Biomedicine
Tropical Medicine