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dc.contributor.authorHaymet, Andrew B
dc.contributor.authorBartnikowski, Nicole
dc.contributor.authorWood, Emily S
dc.contributor.authorVallely, Michael P
dc.contributor.authorMcBride, Angela
dc.contributor.authorYacoub, Sophie
dc.contributor.authorBiering, Scott B
dc.contributor.authorHarris, Eva
dc.contributor.authorSuen, Jacky Y
dc.contributor.authorFraser, John F
dc.date.accessioned2021-08-06T07:57:08Z
dc.date.available2021-08-06T07:57:08Z
dc.date.issued2021
dc.identifier.issn2297-055X
dc.identifier.doi10.3389/fcvm.2021.647086
dc.identifier.urihttp://hdl.handle.net/10072/406665
dc.description.abstractAll human cells are coated by a surface layer of proteoglycans, glycosaminoglycans (GAGs) and plasma proteins, called the glycocalyx. The glycocalyx transmits shear stress to the cytoskeleton of endothelial cells, maintains a selective permeability barrier, and modulates adhesion of blood leukocytes and platelets. Major components of the glycocalyx, including syndecans, heparan sulfate, and hyaluronan, are shed from the endothelial surface layer during conditions including ischaemia and hypoxia, sepsis, atherosclerosis, diabetes, renal disease, and some viral infections. Studying mechanisms of glycocalyx damage in vivo can be challenging due to the complexity of immuno-inflammatory responses which are inextricably involved. Previously, both static as well as perfused in vitro models have studied the glycocalyx, and have reported either imaging data, assessment of barrier function, or interactions of blood components with the endothelial monolayer. To date, no model has simultaneously incorporated all these features at once, however such a model would arguably enhance the study of vasculopathic processes. This review compiles a series of current in vitro models described in the literature that have targeted the glycocalyx layer, their limitations, and potential opportunities for further developments in this field.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherFrontiers Media SA
dc.relation.ispartofpagefrom647086
dc.relation.ispartofjournalFrontiers in Cardiovascular Medicine
dc.relation.ispartofvolume8
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchImmunology
dc.subject.fieldofresearchcode3107
dc.subject.fieldofresearchcode3204
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsCardiac & Cardiovascular Systems
dc.subject.keywordsCardiovascular System & Cardiology
dc.subject.keywordsendothelium
dc.titleStudying the Endothelial Glycocalyx in vitro: What Is Missing?
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationHaymet, AB; Bartnikowski, N; Wood, ES; Vallely, MP; McBride, A; Yacoub, S; Biering, SB; Harris, E; Suen, JY; Fraser, JF, Studying the Endothelial Glycocalyx in vitro: What Is Missing?, Frontiers in Cardiovascular Medicine, 2021, 8, pp. 647086
dcterms.dateAccepted2021-03-19
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.date.updated2021-08-06T00:09:54Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2021 Haymet, Bartnikowski, Wood, Vallely, McBride, Yacoub, Biering, Harris, Suen and Fraser. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
gro.hasfulltextFull Text
gro.griffith.authorFraser, John F.


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