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dc.contributor.authorT., Gedrangeen_US
dc.contributor.authorSpassov, Aen_US
dc.contributor.authorT, Gredesen_US
dc.contributor.authorS, Pavlovicen_US
dc.contributor.authorMack, Florianen_US
dc.contributor.authorMack, Heikeen_US
dc.contributor.authorC, Kunert-Keilen_US
dc.date.accessioned2017-04-24T12:47:50Z
dc.date.available2017-04-24T12:47:50Z
dc.date.issued2009en_US
dc.date.modified2013-09-01T22:54:49Z
dc.identifier.urihttp://hdl.handle.net/10072/40667
dc.description.abstractObjective: The lack of dystrophin in the mouse mutant mdx leads to muscle degeneration and is the most used animal model for Duchenne muscular dystrophy (DMD). The DMD patients have distorted dentofacial morphology which could be a result of changed masticatory mechanics due to muscular damage and dysfunction. The aim of this study is to investigate the regeneration process in the masticatory muscles of mdx-mouse due to the analysis of mRNA expression of the Myosin heavy chain (MyHC) isoforms. METHODS: In a mouse model (mdx and controls; 100 days old, n=10 each group) we examined myosin heavy chain - isoforms expression mRNA in the masticatory mucles and taking samples from masseter (MAS), temporalis (TEM) and tongue (TON) muscles. RESULTS: In control mice IIx isoforms are more expressed as IIb in MAS (65% vs. 30%, p<0.05) and TEM (83% vs. 12%, p<0.05), in TON that relation being inversed (26% vs. 72%, p<0.05). The fiber type distribution in MAS did not change in mdx, as compared to the controls although slight insignificant increase in IIb was observed. In TEM the content of IIb MyHC isoforms was reduced in comparison to the controls (12% resp. 37%; p < 0.01). The TON muscle in the mdx group represents a strongly reduced expression of IIx (26%; controls 31%, p<0.05) and IIb isoforms (74%; controls 69%, p<0.05). All masticatory muscles appeared to have very small amounts of type IIa and no content of type I. These findings were also confirmed by immunohistochemistry and Western blot analysis. CONCLUSION: The observed down regulation of the expression of IIx and IIb MyHC isoforms is an evidence for muscle adaptation process due to dystrophin absence and may be responsible for functional misbalance of masticatory muscles resulting in morphological changes that are observed in DMD patients.en_US
dc.description.publicationstatusYesen_US
dc.languageEnglishen_US
dc.language.isoen_US
dc.publisherNo data provideden_US
dc.publisher.urihttp://iadr.confex.com/iadr/2009miami/webprogram/Paper115739.htmlen_US
dc.relation.ispartofstudentpublicationNen_US
dc.relation.ispartofconferencenameIADR 2009en_US
dc.relation.ispartofconferencetitle87th General Session & Exhibition of the IADRen_US
dc.relation.ispartofdatefrom2009-03-01en_US
dc.relation.ispartofdateto2009-03-04en_US
dc.relation.ispartoflocationMiami, USAen_US
dc.rights.retentionYen_US
dc.subject.fieldofresearchMedical Microbiology not elsewhere classifieden_US
dc.subject.fieldofresearchcode110899en_US
dc.titleRT-PCR quantification of mRNA Myosin isoforms in muscle of mdx-miceen_US
dc.typeConference outputen_US
dc.type.descriptionE3 - Conference Publications (Extract Paper)en_US
dc.type.codeE - Conference Publicationsen_US
gro.date.issued2009
gro.hasfulltextNo Full Text


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