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dc.contributor.authorMuda, Noor Marian
dc.contributor.authorNasreen, Marufa
dc.contributor.authorDhouib, Rabeb
dc.contributor.authorHosmer, Jennifer
dc.contributor.authorHill, Julian
dc.contributor.authorMahawar, Manish
dc.contributor.authorSchirra, Horst Joachim
dc.contributor.authorMcEwan, Alastair G
dc.contributor.authorKappler, Ulrike
dc.date.accessioned2021-08-10T04:46:57Z
dc.date.available2021-08-10T04:46:57Z
dc.date.issued2019
dc.identifier.issn2049-632X
dc.identifier.doi10.1093/femspd/ftz015
dc.identifier.urihttp://hdl.handle.net/10072/406739
dc.description.abstractNon-typeable Haemophilus influenzae (NTHi) is a major pathogen in upper and lower respiratory tract infections in humans, and is increasingly also associated with invasive disease. We have examined two unrelated NTHi invasive disease isolates, R2866 and C188, in order to identify metabolic and physiological properties that distinguish them from respiratory tract disease isolates such as Hi2019. While the general use of the Hi metabolic network was similar across all three strains, the two invasive isolates secreted increased amounts of succinate, which can have anti-inflammatory properties. In addition, they showed a common shift in their carbon source utilization patterns, with strongly enhanced metabolism of nucleoside substrates, glucose and sialic acid. The latter two are major compounds present in blood and cerebrospinal fluid (CSF). Interestingly, C188 and R2866 also shared a reduced ability to invade or survive intracellularly in 16HBE14 bronchial epithelial cells relative to Hi2019 (4-fold (4 h), 25-fold (24 h) reduction). Altered metabolic properties, such as the ones observed here, could arise from genomic adaptations that NTHi undergo during infection. Together these data indicate that shifts in substrate preferences in otherwise conserved metabolic pathways may underlie strain niche specificity and thus have the potential to alter the outcomes of host–NTHi interactions.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherOxford University Press
dc.relation.ispartofpagefromftz015
dc.relation.ispartofissue2
dc.relation.ispartofjournalPathogens and Disease
dc.relation.ispartofvolume77
dc.subject.fieldofresearchCardiovascular medicine and haematology
dc.subject.fieldofresearchImmunology
dc.subject.fieldofresearchMedical microbiology
dc.subject.fieldofresearchMedical biochemistry and metabolomics
dc.subject.fieldofresearchcode3201
dc.subject.fieldofresearchcode3204
dc.subject.fieldofresearchcode3207
dc.subject.fieldofresearchcode3205
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsImmunology
dc.subject.keywordsInfectious Diseases
dc.subject.keywordsMicrobiology
dc.titleMetabolic analyses reveal common adaptations in two invasive Haemophilus influenzae strains
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationMuda, NM; Nasreen, M; Dhouib, R; Hosmer, J; Hill, J; Mahawar, M; Schirra, HJ; McEwan, AG; Kappler, U, Metabolic analyses reveal common adaptations in two invasive Haemophilus influenzae strains, Pathogens and Disease, 2019, 77 (2), pp. ftz015
dcterms.dateAccepted2019-03-19
dc.date.updated2021-08-09T22:27:25Z
dc.description.versionAccepted Manuscript (AM)
gro.rights.copyright© 2019 FEMS. This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Pathogens and Disease following peer review. The definitive publisher-authenticated version Metabolic analyses reveal common adaptations in two invasive Haemophilus influenzae strains, Pathogens and Disease, 2019, 77 (2), pp. ftz015 is available online at: https://doi.org/10.1093/femspd/ftz015.
gro.hasfulltextFull Text
gro.griffith.authorSchirra, Horst Joachim


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