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dc.contributor.authorTa, Hang T
dc.contributor.authorLi, Zhen
dc.contributor.authorHagemeyer, Christoph E
dc.contributor.authorCowin, Gary
dc.contributor.authorZhang, Shaohua
dc.contributor.authorPalasubramaniam, Jathushan
dc.contributor.authorAlt, Karen
dc.contributor.authorWang, Xiaowei
dc.contributor.authorPeter, Karlheinz
dc.contributor.authorWhittaker, Andrew K
dc.description.abstractMagnetic resonance imaging (MRI) is a powerful and indispensable tool in medical research, clinical diagnosis, and patient care due to its high spatial resolution and non-limited penetration depth. The simultaneous use of positive and negative MRI imaging that employs the same contrast agents will significantly improve detection accuracy. Here we report the development of functional multimodal iron oxide nanoparticles for targeted MRI of atherothrombosis using a combination of chemical and biological conjugation techniques. Monodisperse, water-soluble and biocompatible ultra-small magnetic dual contrast iron oxide nanoparticles (DCIONs) were generated using a high-temperature co-precipitation route and appeared to be efficient positive and negative dual contrast agents for magnetic resonance imaging. Using a unique chemo-enzymatic approach involving copper-free click chemistry and Staphylococcus aureus sortase A enzyme conjugation, DCIONs were functionalized with single-chain antibodies (scFv) directed against activated platelets for targeting purposes. The DCIONs were also labelled with fluorescent molecules to allow for optical imaging. The antigen binding activity of the scFv was retained and resulted in the successful targeting of contrast agents to thrombosis as demonstrated in a range of in vitro and in vivo experiments. T1- and T2-weighted MRI of thrombi was recorded and demonstrated the great potential of dual T1/T2 contrast iron oxide particles in imaging of cardiovascular disease.
dc.subject.fieldofresearchBiomedical imaging
dc.subject.keywordsScience & Technology
dc.subject.keywordsEngineering, Biomedical
dc.subject.keywordsMaterials Science, Biomaterials
dc.titleMolecular imaging of activated platelets via antibody-targeted ultra-small iron oxide nanoparticles displaying unique dual MRI contrast
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationTa, HT; Li, Z; Hagemeyer, CE; Cowin, G; Zhang, S; Palasubramaniam, J; Alt, K; Wang, X; Peter, K; Whittaker, AK, Molecular imaging of activated platelets via antibody-targeted ultra-small iron oxide nanoparticles displaying unique dual MRI contrast, Biomaterials, 2017, 134, pp. 31-42
gro.hasfulltextNo Full Text
gro.griffith.authorTa, Hang

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