A proof‐of‐concept study using numerical simulations of an acoustic spheroid‐on‐a‐chip platform for improving 3d cell culture
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Author(s)
Yahyazadeh Shourabi, A
Salajeghe, R
Barisam, M
Kashaninejad, N
Griffith University Author(s)
Year published
2021
Metadata
Show full item recordAbstract
Microfluidic lab‐on‐chip devices are widely being developed for chemical and biological studies. One of the most commonly used types of these chips is perfusion microwells for culturing multicellular spheroids. The main challenge in such systems is the formation of substantial necrotic and quiescent zones within the cultured spheroids. Herein, we propose a novel acoustofluidic integrated platform to tackle this bottleneck problem. It will be shown numerically that such an approach is a potential candidate to be implemented to enhance cell viability and shrinks necrotic and quiescent zones without the need to increase the ...
View more >Microfluidic lab‐on‐chip devices are widely being developed for chemical and biological studies. One of the most commonly used types of these chips is perfusion microwells for culturing multicellular spheroids. The main challenge in such systems is the formation of substantial necrotic and quiescent zones within the cultured spheroids. Herein, we propose a novel acoustofluidic integrated platform to tackle this bottleneck problem. It will be shown numerically that such an approach is a potential candidate to be implemented to enhance cell viability and shrinks necrotic and quiescent zones without the need to increase the flow rate, leading to a significant reduction in costly reagents’ consumption in conventional spheroid‐on‐a‐chip platforms. Proof‐of‐concept, designing procedures and numerical simulation are discussed in detail. Additionally, the effects of acoustic and hydrodynamic parameters on the cultured cells are investigated. The results show that by increasing acoustic boundary displacement amplitude (d0), the spheroid’s proliferating zone enlarges greatly. Moreover, it is shown that by implementing d0 = 0.5 nm, the required flow rate to maintain the necrotic zone below 13% will be decreased 12 times compared to non‐acoustic chips.
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View more >Microfluidic lab‐on‐chip devices are widely being developed for chemical and biological studies. One of the most commonly used types of these chips is perfusion microwells for culturing multicellular spheroids. The main challenge in such systems is the formation of substantial necrotic and quiescent zones within the cultured spheroids. Herein, we propose a novel acoustofluidic integrated platform to tackle this bottleneck problem. It will be shown numerically that such an approach is a potential candidate to be implemented to enhance cell viability and shrinks necrotic and quiescent zones without the need to increase the flow rate, leading to a significant reduction in costly reagents’ consumption in conventional spheroid‐on‐a‐chip platforms. Proof‐of‐concept, designing procedures and numerical simulation are discussed in detail. Additionally, the effects of acoustic and hydrodynamic parameters on the cultured cells are investigated. The results show that by increasing acoustic boundary displacement amplitude (d0), the spheroid’s proliferating zone enlarges greatly. Moreover, it is shown that by implementing d0 = 0.5 nm, the required flow rate to maintain the necrotic zone below 13% will be decreased 12 times compared to non‐acoustic chips.
View less >
Journal Title
Sensors
Volume
21
Issue
16
Copyright Statement
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Subject
Analytical chemistry
Environmental sciences
Ecology
Theory of computation
Distributed computing and systems software
Electrical engineering
Circuits and systems
acoustic microfluidics
lab-on-chip
necrotic
quiescent zones
spheroid-on-chip