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dc.contributor.authorThoo, Lester
dc.contributor.authorFahmi, Mochamad Z
dc.contributor.authorZulkipli, Ihsan N
dc.contributor.authorKeasberry, Natasha
dc.contributor.authorIdris, Adi
dc.date.accessioned2021-09-07T06:23:59Z
dc.date.available2021-09-07T06:23:59Z
dc.date.issued2017
dc.identifier.issn1426-3912
dc.identifier.doi10.5114/ceji.2017.70978
dc.identifier.urihttp://hdl.handle.net/10072/407722
dc.description.abstractCarbon dot (Cdot) nanoparticles are an emerging class of carbon nanomaterials with a promising potential for drug delivery and bio imaging applications. Although the interaction between Cdots and non-immune cell types has been well studied, Cdot interactions with macrophages have not been investigated. Exposure of Cdot nanoparticles to J774.1 cells, a murine macrophage cell line, resulted in minimal toxicity, where notable toxicity was only seen with Cdot concentrations higher than 0.5 mg/ ml. Flow cytometric analysis revealed that Cdots prepared from citric acid were internalized at significantly higher levels by macrophages compared with those prepared from bamboo leaves. Interestingly, macrophages preferentially took up phenylboronic acid (PB)-modified nanoparticles. By fluorescence microscopy, strong blue light-specific punctate Cdot fluorescence resembling Cdot structures in the cytosolic space was mostly observed in J774.1 macrophages exposed to PB-modified nanoparticles and not unmodified Cdot nanoparticles. PB binds to sialic acid residues that are overexpressed on diseased cell surfaces. Our findings demonstrate that PB-conjugated Cdots can be taken up by macrophages with low toxicity and high efficiency. These modified Cdots can be used to deliver drugs to suppress or eliminate aberrant immune cells such as macrophages associated with tumors such as tumor-associated macrophages.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherTermedia Publishing House
dc.relation.ispartofpagefrom324
dc.relation.ispartofpageto330
dc.relation.ispartofissue3
dc.relation.ispartofjournalCentral European Journal of Immunology
dc.relation.ispartofvolume42
dc.subject.fieldofresearchImmunology
dc.subject.fieldofresearchcode3204
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordscarbon dots
dc.subject.keywordsmacrophage
dc.titleInteraction and cellular uptake of surface-modified carbon dot nanoparticles by J774.1 macrophages
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationThoo, L; Fahmi, MZ; Zulkipli, IN; Keasberry, N; Idris, A, Interaction and cellular uptake of surface-modified carbon dot nanoparticles by J774.1 macrophages, Central European Journal of Immunology , 2017, 42 (3), pp. 324-330
dcterms.dateAccepted2016-06-13
dc.date.updated2021-09-07T06:21:52Z
gro.hasfulltextNo Full Text
gro.griffith.authorIdris, Adi


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