Show simple item record

dc.contributor.authorSingh, Neetu
dc.contributor.authorKumar, Rajiv
dc.contributor.authorEngwerda, Christian
dc.contributor.authorSacks, David
dc.contributor.authorNylen, Susanne
dc.contributor.authorSundar, Shyam
dc.date.accessioned2021-09-21T05:46:34Z
dc.date.available2021-09-21T05:46:34Z
dc.date.issued2016
dc.identifier.issn1043-4666
dc.identifier.doi10.1016/j.cyto.2016.06.013
dc.identifier.urihttp://hdl.handle.net/10072/408184
dc.description.abstractThe pro-inflammatory cytokine tumor necrosis factor (TNF)-α has an important role in control of experimental Leishmania donovani infection. Less is known about the role of TNF-α in human visceral leishmaniasis (VL). Evidence for a protective role is primarily based on case reports of VL development in individuals treated with TNF-α neutralizing antibody. In this study, we have evaluated how TNF-α neutralization affects parasite replication and cytokine production in ex vivo splenic aspirates (SA) from active VL patients. The effect of TNF-α neutralization on cell mediated antigen specific responses were also evaluated using whole blood cultures. Neutralization of TNF-α did not affect parasite numbers in SA cultures. Interferon (IFN)-γ levels were significantly reduced, but interleukin (IL)-10 levels were unchanged in these cultures. Leishmania antigen stimulated SA produced significant TNF-α which suggests that TNF-α is actively produced in VL spleen. Further it stimulates IFN-γ production, but no direct effect on parasite replication.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherElsevier
dc.relation.ispartofpagefrom184
dc.relation.ispartofpageto190
dc.relation.ispartofjournalCytokine
dc.relation.ispartofvolume85
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchGenetics
dc.subject.fieldofresearchImmunology
dc.subject.fieldofresearchcode3101
dc.subject.fieldofresearchcode3105
dc.subject.fieldofresearchcode3204
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsBiochemistry & Molecular Biology
dc.subject.keywordsCell Biology
dc.subject.keywordsImmunology
dc.titleTumor necrosis factor alpha neutralization has no direct effect on parasite burden, but causes impaired IFN-γ production by spleen cells from human visceral leishmaniasis patients
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationSingh, N; Kumar, R; Engwerda, C; Sacks, D; Nylen, S; Sundar, S, Tumor necrosis factor alpha neutralization has no direct effect on parasite burden, but causes impaired IFN-γ production by spleen cells from human visceral leishmaniasis patients, Cytokine, 2016, 85, pp. 184-190
dcterms.dateAccepted2016-06-08
dc.date.updated2021-09-21T05:45:02Z
gro.hasfulltextNo Full Text
gro.griffith.authorEngwerda, Christian R.


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record