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dc.contributor.authorLiu, W
dc.contributor.authorWang, Z
dc.contributor.authorLeng, J
dc.contributor.authorWei, H
dc.contributor.authorRen, S
dc.contributor.authorGong, X
dc.contributor.authorChen, C
dc.contributor.authorWang, Y
dc.contributor.authorZhang, R
dc.contributor.authorLi, W
dc.date.accessioned2021-09-23T04:45:59Z
dc.date.available2021-09-23T04:45:59Z
dc.date.issued2021
dc.identifier.issn0327-9545
dc.identifier.doi10.32604/BIOCELL.2020.013202
dc.identifier.urihttp://hdl.handle.net/10072/408271
dc.description.abstractHeat stress (HS) reaction can lead to serious physiological dysfunction associated with cardiovascular and various organ diseases. Ginsenoside Rg3 (G-Rg3) is a representative component of ginseng rare saponin and can protect against multiple organs, also used as functional food to adjust the balance of the human body, but the therapeutic effect and molecular mechanism of G-Rg3 on male diseases under HS are underexplored. The aim of the present study, G-Rg3 was prepared through the efficient conversion of ginsenoside Rd and investigate the contribution of G-Rg3 to testicular injury induced exposure to HS. All mice were divided into four groups as follows: normal group, HS group, and HS+G-Rg3 (5 and 10 mg/kg) groups. G-Rg3 was administered orally for 14 days, then exposed to a single scrotal heat treatment (43°C, 18min) on the 7th day. After HS treatment, the morphology of testis and epididymis changes, and caused a significant loss of multinucleated giant cells, desquamation of germ cells in destructive seminiferous tubules, and degenerative Leydig cells, further destroying the production of sperm. After administration G-Rg3 (5 and 10 mg/kg/day) for 2 weeks, the spermatogenic-related indexes of testosterone levels and superoxide dismutase (SOD) activity, glutathione (GSH) content significantly (p < 0.01) increase compared with the HS group. Moreover, G-Rg3 treatment effectively ameliorated the production of malondialdehyde (MDA) (p < 0.05 or p < 0.01). Importantly, G-Rg3 exhibited the protective potential against HS-induced injury not only suppressing the protein levels of heme oxygenase-1 (HO-1), hypoxia-inducible factor-1α (HIF-1α), and heat shock protein 70 (HSP70) but also modulating the Bcl-2 family (p < 0.01 or p < 0.001) and activation of mitogen-activated protein kinase (MAPK) signaling pathways (p < 0.01). For most of the parameters tested, the HS+G-Rg3 (10 mg/kg) group exhibited potent effects compared with those exhibited by the low dose (5 mg/kg) group. In conclusion, the present study demonstrated that G-Rg3 exerted protective effects against HS-induced testicular dysfunction via inhibiting the MAPK-mediated oxidative stress and apoptosis in mice.
dc.description.peerreviewedYes
dc.languageen
dc.publisherComputers, Materials and Continua (Tech Science Press)
dc.relation.ispartofpagefrom655
dc.relation.ispartofpageto669
dc.relation.ispartofissue4
dc.relation.ispartofjournalBiocell
dc.relation.ispartofvolume44
dc.subject.fieldofresearchMedical microbiology
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchcode3207
dc.subject.fieldofresearchcode3101
dc.title20(R)-ginsenoside Rg3, a product of high-efficiency thermal deglycosylation of ginsenoside Rd, exerts protective effects against scrotal heat-induced spermatogenic damage in mice
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationLiu, W; Wang, Z; Leng, J; Wei, H; Ren, S; Gong, X; Chen, C; Wang, Y; Zhang, R; Li, W, 20(R)-ginsenoside Rg3, a product of high-efficiency thermal deglycosylation of ginsenoside Rd, exerts protective effects against scrotal heat-induced spermatogenic damage in mice, Biocell, 2021, 44 (4), pp. 655-669
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.date.updated2021-09-22T04:32:59Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© The Author(s) 2021. This work is licensed under a Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorChen, Chen


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