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dc.contributor.authorKasetsiriku, Surasak
dc.contributor.authorKetpun, Dettachai
dc.contributor.authorChuah, Yon Jin
dc.contributor.authorSriphutkiat, Yannapol
dc.contributor.authorWang, Dong-An
dc.contributor.authorZhou, Yufeng
dc.description.abstractBackground: Surface modification is used to modify the biomaterials for the regulation of cell culture using different approaches, such as chemical graft and mechanical treatment. However, those conventional methodologies often require precise fabrication in a high resolution involving either high cost or laborious steps to remove chemical residues that are toxic to the cells. Methods: A novel and simple method was proposed and evaluated to rapidly generate surface ceases on the gelatin methacrylate (gelMA) surface using the heating-hydration process. Human umbilical vein endothelial cells (HUVECs) were cultured on the gelMA surface. The surface binding was characterized using the RGD (Arg-Gly-Asp) antibodies and cell adhesion pattern captured by scanning electron microscopy. The effect of the heating-hydration parameters on the creasing formation was investigated. The morphology of HUVECs cultured on such micropatterned gelMA was characterized and compared. Results: It is found that the hydration solution, gelMA mixture, and hydration rate are the major factors that influence the cracking sizes in the range from 20 to 120 µm which resulted in capillary-like patterns on the gelMA surface. Low concentration of gelMA, high water concentration of cooling agent, and slow hydration rate result in the long creases, and heating of at least 60 min is required for complete dehydration. Strong fluorescence was around the creases with RGD-staining. Consequently, micropatterned gelMA demonstrated good biocompatibility with endothelial cells with more than 95% cell viability and continuous cell proliferation throughout 2 weeks as well as a good trace of neovascular formation. In comparison, normal gelMA surface did not exhibit RGD-fluorescent signals, and the cultured HUVECs on it were rounded with no spreading for network formation. Conclusion: The heating-hydration approach can successfully and easily produce the micropatterned gelMA that allows rapid and effective vascularization to potentially improve the functionalities of the tissue-engineered construct.en_US
dc.publisherSpringer Science and Business Media LLCen_US
dc.relation.ispartofjournalTissue Engineering and Regenerative Medicineen_US
dc.subject.fieldofresearchBiomedical engineeringen_US
dc.subject.fieldofresearchClinical sciencesen_US
dc.titleSurface Creasing-Induced Micropatterned GelMA Using Heating-Hydration Fabrication for Effective Vascularizationen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Articlesen_US
dcterms.bibliographicCitationKasetsiriku, S; Ketpun, D; Chuah, YJ; Sriphutkiat, Y; Wang, D-A; Zhou, Y, Surface Creasing-Induced Micropatterned GelMA Using Heating-Hydration Fabrication for Effective Vascularization, Tissue Engineering and Regenerative Medicine, 2021, 18 (5), pp. 759-773en_US
gro.hasfulltextNo Full Text
gro.griffith.authorKasetsirikul, Surasak

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