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dc.contributor.authorSinha, R
dc.contributor.authorNgo, MD
dc.contributor.authorBartlett, S
dc.contributor.authorBielefeldt-Ohmann, H
dc.contributor.authorKeshvari, S
dc.contributor.authorHasnain, SZ
dc.contributor.authorDonovan, ML
dc.contributor.authorKling, JC
dc.contributor.authorBlumenthal, A
dc.contributor.authorChen, C
dc.contributor.authorShort, KR
dc.contributor.authorRonacher, K
dc.date.accessioned2021-09-30T04:06:49Z
dc.date.available2021-09-30T04:06:49Z
dc.date.issued2021
dc.identifier.issn2235-2988
dc.identifier.doi10.3389/fcimb.2021.691823
dc.identifier.urihttp://hdl.handle.net/10072/408479
dc.description.abstractType 2 diabetes (T2D) is a well-known risk factor for tuberculosis (TB), but little is known about pre-diabetes and the relative contribution of impaired glucose tolerance vs. obesity towards susceptibility to TB. Here, we developed a preclinical model of pre-diabetes and TB. Mice fed a high fat diet (HFD) for 12 weeks presented with impaired glucose tolerance and hyperinsulinemia compared to mice fed normal chow diet (NCD). Infection with M. tuberculosis (Mtb) H37Rv after the onset of dysglycemia was associated with significantly increased lung pathology, lower concentrations of TNF-α, IFN-γ, IFN-β and IL-10 and a trend towards higher bacterial burden at 3 weeks post infection. To determine whether the increased susceptibility of pre-diabetic mice to TB is reversible and is associated with dysglycemia or increased body fat mass, we performed a diet reversal experiment. Pre-diabetic mice were fed a NCD for 10 additional weeks (HFD/NCD) at which point glucose tolerance was restored, but body fat mass remained higher compared to control mice that consumed NCD throughout the entire experiment (NCD/NCD). Upon Mtb infection HFD/NCD mice had significantly lower bacterial burden compared to NCD/NCD mice and this was accompanied by restored IFN-γ responses. Our findings demonstrate that pre-diabetes increases susceptibility to TB, but a high body mass index without dysglycemia is protective. This murine model offers the opportunity to further study the underlying immunological, metabolic and endocrine mechanisms of this association.
dc.description.peerreviewedYes
dc.languageeng
dc.publisherFrontiers Media SA
dc.relation.ispartofpagefrom691823
dc.relation.ispartofjournalFrontiers in Cellular and Infection Microbiology
dc.relation.ispartofvolume11
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchEndocrinology
dc.subject.fieldofresearchVirology
dc.subject.fieldofresearchClinical sciences
dc.subject.fieldofresearchcode3107
dc.subject.fieldofresearchcode320208
dc.subject.fieldofresearchcode310706
dc.subject.fieldofresearchcode3202
dc.subject.keywordsMycobacterium tuberculosis
dc.subject.keywordsdiabetes
dc.subject.keywordsdisease severity
dc.subject.keywordshigh fat diet
dc.subject.keywordsimpaired glucose tolerance
dc.titlePre-Diabetes Increases Tuberculosis Disease Severity, While High Body Fat Without Impaired Glucose Tolerance Is Protective
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationSinha, R; Ngo, MD; Bartlett, S; Bielefeldt-Ohmann, H; Keshvari, S; Hasnain, SZ; Donovan, ML; Kling, JC; Blumenthal, A; Chen, C; Short, KR; Ronacher, K, Pre-Diabetes Increases Tuberculosis Disease Severity, While High Body Fat Without Impaired Glucose Tolerance Is Protective, Frontiers in Cellular and Infection Microbiology, 2021, 11, pp. 691823
dcterms.dateAccepted2021-06-17
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.date.updated2021-09-30T03:59:21Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2021 Sinha, Ngo, Bartlett, Bielefeldt-Ohmann, Keshvari, Hasnain, Donovan, Kling, Blumenthal, Chen, Short and Ronacher. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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gro.griffith.authorChen, Chen


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