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dc.contributor.authorYuan, D
dc.contributor.authorYan, S
dc.contributor.authorZhang, J
dc.contributor.authorGuijt, RM
dc.contributor.authorZhao, Q
dc.contributor.authorLi, W
dc.date.accessioned2021-10-14T00:10:40Z
dc.date.available2021-10-14T00:10:40Z
dc.date.issued2021
dc.identifier.issn0003-2700en_US
dc.identifier.doi10.1021/acs.analchem.1c02389en_US
dc.identifier.urihttp://hdl.handle.net/10072/408864
dc.description.abstractCyanobacteria have a wide range of impact on natural ecosystems, and have been recognized as potentially rich sources of pharmacological and structurally interesting secondary metabolites. To better understand the basic molecular processes and mechanisms that influence and regulate the growth (like length) of cyanobacteria, or connections between environment, genotype, and phenotype, it would be essential to separate shape-synchronized cyanobacterial cell populations with relatively uniform length and size. This work proposes a novel and efficient method to separate cyanobacterial Anabaena by shape (rod aspect ratio) using viscoelastic microfluidics in a straight channel with expansion-contraction cavity arrays (ECCA channel). The biocompatible viscoelastic solutions with dissolved polymer would induce a combined effect of inertial lift force, elastic force, and secondary drag force for Anabaena flowing in it. Therefore, Anabaena with different lengths reach different lateral equilibrium positions and flow out from different outlets. Factors including flow rate, fluid viscoelasticity, channel structure, and length on the shape-based cell separation were studied systematically. This work, for the first time, demonstrates continuous and sheathless shape-based separation of cyanobacteria using viscoelastic microfluidics. Moreover, its ability to manipulate objects with different morphologies and with a size of >100 μm will extend the capability of microfluidics to a completely new field that has never been reached and would be attractive across a range of new applications.en_US
dc.languageengen_US
dc.publisherAmerican Chemical Society (ACS)en_US
dc.relation.ispartofpagefrom12648en_US
dc.relation.ispartofpageto12654en_US
dc.relation.ispartofissue37en_US
dc.relation.ispartofjournalAnalytical Chemistryen_US
dc.relation.ispartofvolume93en_US
dc.titleSheathless Separation of Cyanobacterial Anabaena by Shape Using Viscoelastic Microfluidicsen_US
dc.typeJournal articleen_US
dcterms.bibliographicCitationYuan, D; Yan, S; Zhang, J; Guijt, RM; Zhao, Q; Li, W, Sheathless Separation of Cyanobacterial Anabaena by Shape Using Viscoelastic Microfluidics, Analytical Chemistry, 2021, 93 (37), pp. 12648-12654en_US
dc.date.updated2021-10-06T22:00:25Z
dc.description.versionAccepted Manuscript (AM)en_US
gro.rights.copyrightThis document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, © YEAR American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.analchem.1c02389en_US
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gro.griffith.authorZhang, Jun


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