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  • Antibody coated conductive polymer for the electrochemical immunosensing of Human Cardiac Troponin I in blood plasma

    Author(s)
    Gholami, MD
    O'Mullane, AP
    Sonar, P
    Ayoko, GA
    Izake, EL
    Griffith University Author(s)
    Sonar, Prashant
    Year published
    2021
    Metadata
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    Abstract
    Cardiac troponin I (cTnI) is a sensitive biomarker for cardiovascular disease (CVD). Rapid determination of cTnI concentration in blood can greatly reduce the potential of significant heart damage and heart failure. Herein, we demonstrate a new electrochemical immunosensor for selective affinity binding and rapid detection of cTnI in blood plasma by an electrochemical method. A conductive film of “poly 2,5-bis(2-thienyl)3,4-diamine-terthiophene (PDATT)” was deposited onto an Indium Tin Oxide (ITO) electrode using chronoamperometry. Anti-cardiac troponin I antibody was then attached to the two amine (NH2) groups substituted ...
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    Cardiac troponin I (cTnI) is a sensitive biomarker for cardiovascular disease (CVD). Rapid determination of cTnI concentration in blood can greatly reduce the potential of significant heart damage and heart failure. Herein, we demonstrate a new electrochemical immunosensor for selective affinity binding and rapid detection of cTnI in blood plasma by an electrochemical method. A conductive film of “poly 2,5-bis(2-thienyl)3,4-diamine-terthiophene (PDATT)” was deposited onto an Indium Tin Oxide (ITO) electrode using chronoamperometry. Anti-cardiac troponin I antibody was then attached to the two amine (NH2) groups substituted on the central thiophene of terthiophene repeating unit of the polymer chain via amide bond formation. The gaps on the surface of the antibody coated immunosensor were backfilled with bovine serum albumin (BSA) to prevent nonspecific binding of interfering molecules. Differential pulse voltammetry (DPV) was used to determine cTnI upon the formation of cTnI immunocomplex on the sensing surface, appearing a peak at 0.27 V. The response range was 0.01–100 ng mL−1 with limit of quantification down to 0.01 ng mL−1. The developed immunosensor was used to determine cTnI in spiked blood plasma without interference from cardiac troponin T (cTnT). Therefore, this new sensor can be utilised for the detection of cTnI biomarker in pathological laboratories and points of care in less than 15 min.
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    Journal Title
    Analytica Chimica Acta
    Volume
    1185
    DOI
    https://doi.org/10.1016/j.aca.2021.339082
    Subject
    Analytical biochemistry
    Analytical chemistry
    Other chemical sciences
    Publication URI
    http://hdl.handle.net/10072/408999
    Collection
    • Journal articles

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