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dc.contributor.authorGholami, MD
dc.contributor.authorO'Mullane, AP
dc.contributor.authorSonar, P
dc.contributor.authorAyoko, GA
dc.contributor.authorIzake, EL
dc.date.accessioned2021-10-13T04:16:48Z
dc.date.available2021-10-13T04:16:48Z
dc.date.issued2021
dc.identifier.issn0003-2670
dc.identifier.doi10.1016/j.aca.2021.339082
dc.identifier.urihttp://hdl.handle.net/10072/408999
dc.description.abstractCardiac troponin I (cTnI) is a sensitive biomarker for cardiovascular disease (CVD). Rapid determination of cTnI concentration in blood can greatly reduce the potential of significant heart damage and heart failure. Herein, we demonstrate a new electrochemical immunosensor for selective affinity binding and rapid detection of cTnI in blood plasma by an electrochemical method. A conductive film of “poly 2,5-bis(2-thienyl)3,4-diamine-terthiophene (PDATT)” was deposited onto an Indium Tin Oxide (ITO) electrode using chronoamperometry. Anti-cardiac troponin I antibody was then attached to the two amine (NH2) groups substituted on the central thiophene of terthiophene repeating unit of the polymer chain via amide bond formation. The gaps on the surface of the antibody coated immunosensor were backfilled with bovine serum albumin (BSA) to prevent nonspecific binding of interfering molecules. Differential pulse voltammetry (DPV) was used to determine cTnI upon the formation of cTnI immunocomplex on the sensing surface, appearing a peak at 0.27 V. The response range was 0.01–100 ng mL−1 with limit of quantification down to 0.01 ng mL−1. The developed immunosensor was used to determine cTnI in spiked blood plasma without interference from cardiac troponin T (cTnT). Therefore, this new sensor can be utilised for the detection of cTnI biomarker in pathological laboratories and points of care in less than 15 min.
dc.description.peerreviewedYes
dc.languageen
dc.publisherElsevier BV
dc.relation.ispartofpagefrom339082
dc.relation.ispartofjournalAnalytica Chimica Acta
dc.relation.ispartofvolume1185
dc.subject.fieldofresearchAnalytical biochemistry
dc.subject.fieldofresearchAnalytical chemistry
dc.subject.fieldofresearchOther chemical sciences
dc.subject.fieldofresearchcode310101
dc.subject.fieldofresearchcode3401
dc.subject.fieldofresearchcode3499
dc.titleAntibody coated conductive polymer for the electrochemical immunosensing of Human Cardiac Troponin I in blood plasma
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationGholami, MD; O'Mullane, AP; Sonar, P; Ayoko, GA; Izake, EL, Antibody coated conductive polymer for the electrochemical immunosensing of Human Cardiac Troponin I in blood plasma, Analytica Chimica Acta, 2021, 1185, pp. 339082
dc.date.updated2021-10-08T00:34:46Z
gro.hasfulltextNo Full Text
gro.griffith.authorSonar, Prashant


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