dc.contributor.author | Wong, MB | |
dc.contributor.author | Noe, N | |
dc.contributor.author | Richter-Landsberg, C | |
dc.contributor.author | Pountney, DL | |
dc.date.accessioned | 2017-05-03T15:03:40Z | |
dc.date.available | 2017-05-03T15:03:40Z | |
dc.date.issued | 2010 | |
dc.date.modified | 2011-09-22T06:49:26Z | |
dc.identifier.issn | 0022-3042 | |
dc.identifier.doi | 10.1111/j.1471-4159.2010.06943.x | |
dc.identifier.uri | http://hdl.handle.net/10072/40899 | |
dc.description.abstract | Many neurodegenerative diseases are characterised by microscopically- visible protein aggregates, or inclusion bodies, within neural cells. The ubiquitin homologue, SUMO-1, has been identified in sub-domains of pathological inclusion bodies in several neurodegenerative diseases. Inclusion bodies are believed to be formed actively in a defensive response to soluble cytotoxic protein aggregates. We hypothesised that SUMO-1 may become associated with lysosomes in this response. Protein aggregation was induced in 1321N1 glioma cells by proteasome inhibition with MG132 or transient transfection with Q74-EGFP. Fluorescence immunohistochemistry identified co-localisation of SUMO-1 and the lysosomal marker, cathepsin D, in both the transfected cells and MG132 treated cells, increasing over time at 24-96 h post-transfection/ treatment. SUMO-1-positive lysosomes were also detected following MG132-treatment of 1321N1 cells expressing SUMO-1-GFP and stained with Lysotracker dye. SUMO-1 did not mark lysosomes in untransfected or sham treated cells. To determine if SUMO-1 also marks lysosomes in disease, we examined 5 cases of progressive supranuclear palsy (PSP) and 5 cases of MSA. Punctate co-localisation of cathepsin D and SUMO-1 was consistently associated with both the tau-positive PSP inclusions and the a-synuclein-positive MSA inclusions. A similar pattern was also found with the OLN-t40 oligodendrocyte model. These findings suggest a role for SUMO-1 in the autophagy-lysosome pathway linked to the response to protein aggregates. | |
dc.description.publicationstatus | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Wiley | |
dc.publisher.place | United Kingdom | |
dc.relation.ispartofstudentpublication | Y | |
dc.relation.ispartofconferencename | 10th Biennial Meeting of the Asia-Pacific Society of Neurochemistry | |
dc.relation.ispartofconferencetitle | JOURNAL OF NEUROCHEMISTRY | |
dc.relation.ispartofdatefrom | 2010-10-18 | |
dc.relation.ispartofdateto | 2010-10-18 | |
dc.relation.ispartoflocation | Thailand | |
dc.relation.ispartofpagefrom | 44 | |
dc.relation.ispartofpageto | 44 | |
dc.relation.ispartofvolume | 115 | |
dc.rights.retention | Y | |
dc.subject.fieldofresearch | Biochemistry and cell biology | |
dc.subject.fieldofresearch | Cell neurochemistry | |
dc.subject.fieldofresearch | Neurosciences | |
dc.subject.fieldofresearchcode | 3101 | |
dc.subject.fieldofresearchcode | 310104 | |
dc.subject.fieldofresearchcode | 3209 | |
dc.title | SUMO-1 Marks Lysosomes in Neurodegenerative Diseases and in the Cellular Response to Aggregated Proteins | |
dc.type | Conference output | |
dc.type.description | E3 - Conferences (Extract Paper) | |
dc.type.code | E - Conference Publications | |
gro.date.issued | 2010 | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Pountney, Dean L. | |