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  • Involvement of prolyl isomerase PIN1 in the cell cycle progression and proliferation of hepatic oval cells

    Author(s)
    Risal, Prabodh
    Shrestha, Nirajan
    Chand, Lokendra
    Sylvester, Karl G
    Jeong, Yeon Jun
    Griffith University Author(s)
    Shrestha, Nirajan
    Year published
    2017
    Metadata
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    Abstract
    Liver regenerates remarkably after toxic injury or surgical resection. In the case of failure of resident hepatocytes to restore loss, repopulation is carried out by induction, proliferation, and differentiation of the progenitor cell. Although, some signaling pathways have been verified to contribute oval cell-mediated liver regeneration, role of Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1(Pin1) in the oval cells proliferation is unknown. In the present study, we evaluate the role of Pin1 in oval cells proliferation. In our study, the expression of Pin1 in the mice liver increased after three weeks feeding of 3, ...
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    Liver regenerates remarkably after toxic injury or surgical resection. In the case of failure of resident hepatocytes to restore loss, repopulation is carried out by induction, proliferation, and differentiation of the progenitor cell. Although, some signaling pathways have been verified to contribute oval cell-mediated liver regeneration, role of Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1(Pin1) in the oval cells proliferation is unknown. In the present study, we evaluate the role of Pin1 in oval cells proliferation. In our study, the expression of Pin1 in the mice liver increased after three weeks feeding of 3, 5-diethoxycarbonyl-1, 4-dihydrocollidine (DDC) diet along with the proliferation of oval cells. The expression of Pin1 was higher in oval cells compared to the hepatocytes.Pin1 inhibition by Juglone reduced oval cell proliferation, which was restored to normal when oval cells were treated with IGF-1. Consistent with increased cell growth, expression of Pin1, β-catenin and PCNA were increased in IGF-1 treated cells in a time dependent manner. In FACS analysis, siRNA-mediated knockdown of the Pin1 protein in the oval cells significantly increased the numbers of cells in G0/G1 phase. Furthermore, hepatocyte when treated with TGF-β showed marked reduction in cell proliferation and expression of Pin1 whereas this effect was not seen in the oval cells treated with TGF-β. In conclusion, Pin1 plays important role in the cell cycle progression and increase oval cells proliferation which may be crucial in chronic liver injury.
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    Journal Title
    Pathology - Research and Practice
    Volume
    213
    Issue
    4
    DOI
    https://doi.org/10.1016/j.prp.2017.01.005
    Subject
    Clinical sciences
    Science & Technology
    Life Sciences & Biomedicine
    Pathology
    3, 5-Diethoxycarbonyl-1
    4-Dihydrocollidine
    Publication URI
    http://hdl.handle.net/10072/409060
    Collection
    • Journal articles

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