Show simple item record

dc.contributor.authorShrestha, Nirajan
dc.contributor.authorChand, Lokendra
dc.contributor.authorHan, Myung Kwan
dc.contributor.authorLee, Seung Ok
dc.contributor.authorKim, Chan Young
dc.contributor.authorJeong, Yeon Jun
dc.date.accessioned2021-10-17T06:40:53Z
dc.date.available2021-10-17T06:40:53Z
dc.date.issued2016
dc.identifier.issn0278-6915
dc.identifier.doi10.1016/j.fct.2016.04.024
dc.identifier.urihttp://hdl.handle.net/10072/409134
dc.description.abstractGlutamine, traditionally a non-essential amino acid, now has been considered as essential in serious illness and injury. It is a major precursor for glutathione synthesis. However, the anti-fibrotic effect of glutamine and its molecular mechanism in experimental liver fibrosis have not been explored. In the present study we aimed to examine the potential role of glutamine in carbon tetrachloride (CCl4) induced liver fibrosis and TGF-β1 mediated epithelial mesenchymal transition (EMT) and apoptosis in mouse hepatocytes. Liver fibrosis was induced by intraperitoneal injection of CCl4 three times a week for 10 weeks. Glutamine treatment effectively attenuated liver injury and oxidative stress. Collagen content was significantly decreased in liver sections of glutamine treated mice compared to CCl4 model mice. Furthermore, glutamine decreased expression level of α-SMA and TGF-β in liver tissue. Our in vitro study showed that TGF-β1 treatment in hepatocytes resulted in loss of E-cadherin and increased expression of mesenchymal markers and EMT related transcription factor. In addition, TGF-β1 increased the expression of apoptotic markers. However, glutamine interestingly suppressed TGF-β1 mediated EMT and apoptosis. In conclusion, our results suggest that glutamine ameliorates CCl4 induced liver fibrosis and suppresses TGF-β1 induced EMT progression and apoptosis.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherElsevier
dc.relation.ispartofpagefrom129
dc.relation.ispartofpageto137
dc.relation.ispartofjournalFood and Chemical Toxicology
dc.relation.ispartofvolume93
dc.subject.fieldofresearchFood sciences
dc.subject.fieldofresearchcode3006
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsFood Science & Technology
dc.subject.keywordsToxicology
dc.subject.keywordsCarbon tetrachloride
dc.titleGlutamine inhibits CCl4 induced liver fibrosis in mice and TGF-β1 mediated epithelial–mesenchymal transition in mouse hepatocytes
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationShrestha, N; Chand, L; Han, MK; Lee, SO; Kim, CY; Jeong, YJ, Glutamine inhibits CCl4 induced liver fibrosis in mice and TGF-β1 mediated epithelial–mesenchymal transition in mouse hepatocytes, Food and Chemical Toxicology, 2016, 93, pp. 129-137
dcterms.dateAccepted2016-04-27
dc.date.updated2021-10-17T06:39:17Z
gro.hasfulltextNo Full Text
gro.griffith.authorShrestha, Nirajan


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record