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dc.contributor.authorOmer, E
dc.contributor.authorElshamy, A
dc.contributor.authorTaher, R
dc.contributor.authorEl-Kashak, W
dc.contributor.authorShalom, J
dc.contributor.authorWhite, A
dc.contributor.authorCock, I
dc.description.abstractIntroduction: Exposure to high levels of antioxidants has been linked to the treatment and prevention of some cancers. Although Cakile maritima has a high antioxidant capacity, it is yet to be tested for the ability to inhibit the proliferation of cancer cells. Methods: Solvent extracts prepared from C. maritima plant material were analysed for antioxidant capacity by the DPPH free radical scavenging assay. Anti-proliferative activities against CaCo2 and HeLa cancer cells were determined by an MTS based cell proliferation assay. Toxicity was determined by the Artemia franciscana bioassay. The most potent anti-proliferative extract (hexane) was further investigated using non-targeted GC-MS headspace analysis. Results: Good DPPH radical scavenging activity was calculated for all C. maritima extracts. The methanolic and ethyl acetate extracts had particularly strong antioxidant activity (IC 50 of 4.7 and 3.4 μg/mL respectively). Interestingly, the hexane extract which had the lowest DPPH radical scavenging activity (IC 50 13.6 μg/mL), was the most potent inhibitor or CaCo2 and HeLa carcinoma cell growth, with IC 50 's of 12 and 126 μg/mL respectively. The ethyl acetate extract was also a potent inhibitor of proliferation (IC 50 values of 185 and 468 μg/mL against CaCo2 and HeLa, respectively). The methanolic extract (IC 50 values of 2261 and 2046 μg/mL against CaCo2 and HeLa respectively) displayed only moderate anti-proliferative activity, demonstrating that antioxidant activity did not correspond with anti-proliferative activity. All of the extracts were determined to be nontoxic in the Artemia franciscana bioassay, with LC50 values substantially >1000 μg/mL. Non-biased GC-MS headspace analysis of the C. maritima hexane extract highlighted several interesting compounds that may contribute to the therapeutic bioactivities of the extract. Conclusion: The lack of toxicity and the anti-proliferative activity of the hexane and ethyl acetate C. maritima extracts against HeLa and CaCo2 cancer cell lines indicates their potential in the treatment and prevention of some cancers.
dc.publisherEManuscript Technologies
dc.relation.ispartofjournalPharmacognosy Journal
dc.subject.fieldofresearchPlant biology
dc.subject.fieldofresearchTraditional, complementary and integrative medicine
dc.subject.fieldofresearchPharmacology and pharmaceutical sciences
dc.titleCakile maritima scop. extracts inhibit CaCo2 and HeLa human carcinoma cell growth: GC-MS analysis of an anti-proliferative extract
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationOmer, E; Elshamy, A; Taher, R; El-Kashak, W; Shalom, J; White, A; Cock, I, Cakile maritima scop. extracts inhibit CaCo2 and HeLa human carcinoma cell growth: GC-MS analysis of an anti-proliferative extract, Pharmacognosy Journal, 2019, 11 (2), pp. 258-266
gro.hasfulltextNo Full Text
gro.griffith.authorCock, Ian E.

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