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dc.contributor.authorIdris, Adi
dc.contributor.authorZulkipli, Ihsan N
dc.contributor.authorZulhilmi, Nurul Ramizah
dc.contributor.authorLee, Huan F
dc.contributor.authorRajabalaya, Rajan
dc.contributor.authorChee, Lim Y
dc.contributor.authorMajid, Mohamed
dc.contributor.authorDavid, Sheba R
dc.description.abstractBackground: Melastoma malabathricum (MM) is a traditional plant used in the Borneo region. The cytotoxic effects of methanol extracts from MM leaves have been reported in a number of human cancer cell lines. However, the mode of cell death by MM has not been investigated. Objective: We investigated the cytotoxic effects of MM in both human breast and lung cancer cell lines, MCF-7 and A549, respectively, and defined the mode of cell death. Materials and Methods: Cell viability was measured using the 3-(4-, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Annexin-V/propidium iodide (PI) and terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining was done to determine the mode of cell death. Results: The MTT assay revealed that MM extract had an IC50 of >400 μ g/ml on both cell lines at 24 h posttreatment. Flow cytometric and fluorescence microscopy analysis of Annexin-V/PI stained MM-treated cells revealed that the majority of the cells underwent secondary necrosis/late apoptosis. TUNEL assay showed that little to no DNA nicks were present in MM-treated cells, suggesting that cells have undergone secondary necrosis, not late apoptosis, at that time point. Conclusion: MCF-7 and A549 cells undergoes secondary necrosis 24 h post-treatment with MM extract. MM leaf extract could be a potential source for a novel anti-tumor agent for cancer therapy.
dc.relation.ispartofjournalPharmacognosy Magazine
dc.subject.fieldofresearchPharmacology and pharmaceutical sciences
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsChemistry, Medicinal
dc.subject.keywordsPharmacology & Pharmacy
dc.titleMelastoma malabathricum Ethyl Acetate Fraction Induces Secondary Necrosis in Human Breast and Lung Cancer Cell Lines
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationIdris, A; Zulkipli, IN; Zulhilmi, NR; Lee, HF; Rajabalaya, R; Chee, LY; Majid, M; David, SR, Melastoma malabathricum Ethyl Acetate Fraction Induces Secondary Necrosis in Human Breast and Lung Cancer Cell Lines, Pharmacognosy Magazine, 2017, 13 (51), pp. S688-S692
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gro.griffith.authorIdris, Adi

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