Show simple item record

dc.contributor.authorBin Hamid, Faysal
dc.contributor.authorLu, Cu-Tai
dc.contributor.authorMatos, Marco
dc.contributor.authorCheng, Tracie
dc.contributor.authorGopalan, Vinod
dc.contributor.authorLam, Alfred King-yin
dc.date.accessioned2021-11-01T05:54:20Z
dc.date.available2021-11-01T05:54:20Z
dc.date.issued2021
dc.identifier.issn2210-7762
dc.identifier.doi10.1016/j.cancergen.2021.02.002
dc.identifier.urihttp://hdl.handle.net/10072/409383
dc.description.abstractBackground: The purposes of the study were to enumerate and characterise the circulating tumour cell (CTC) and cluster/micro-emboli (CTM) in blood from patients with colorectal carcinoma (CRC) as well as to investigate their clinical relevance. Methods: Peripheral blood of six healthy donors (control) and sixty-two patients with CRC were collected to isolate CTCs by an immunomagnetic negative selection approach. EPCAM and cytokeratin 18 (CK18) antibodies were used to identify the CTCs. The size and the phenotypic variations were evaluated to characterise these isolated CTCs. Additionally, mRNA expressions of the CTCs and the corresponding primary carcinoma were assessed using a multi-gene panel to determine the cellular heterogeneities between CTCs and primary carcinoma. Results: We detected CTCs and CTMs in 72% (41/57) and 32% (18/57) of the patients with CRC, respectively. The total number and length were significantly higher (p<0.0001) in the CTCs than the CTMs. CTCs, especially EPCAMPositiveCK18Posositve subclones, were detected more in the patients with advanced pathological cancer stages when compared to those with early cancer stages (mean: 12.5 vs 4.0, p=0.0068). mRNA profiling of CTCs unveiled three different CTC subtypes expressing epithelial, epithelium-mesenchymal transition (EMT) and stemness signatures, which were different from those of the primary carcinoma. The expressions of EPCAM, HRAS, BRAF, TP53, SLUG, TWIST1, CD44 and MMP9 of CTCs were altered when compared to the primary tumours in patients with CRC. Conclusion: Our findings provide insights into the biology of the CTC, presence of heterogeneous CTC populations in CRC and differential expression of genes in different pathological stages of CTC which can improve the management of patients with CRC.
dc.description.peerreviewedYes
dc.languageEnglish
dc.publisherElsevier
dc.relation.ispartofpagefrom48
dc.relation.ispartofpageto57
dc.relation.ispartofjournalCancer Genetics
dc.relation.ispartofvolume254
dc.subject.fieldofresearchOncology and carcinogenesis
dc.subject.fieldofresearchcode3211
dc.subject.keywordsScience & Technology
dc.subject.keywordsLife Sciences & Biomedicine
dc.subject.keywordsOncology
dc.subject.keywordsGenetics & Heredity
dc.subject.keywordsCirculating tumour cells
dc.titleEnumeration, characterisation and clinicopathological significance of circulating tumour cells in patients with colorectal carcinoma
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationBin Hamid, F; Lu, C-T; Matos, M; Cheng, T; Gopalan, V; Lam, AK-Y, Enumeration, characterisation and clinicopathological significance of circulating tumour cells in patients with colorectal carcinoma, Cancer Genetics, 2021, 254, pp. 48-57
dcterms.dateAccepted2021-02-07
dcterms.licensehttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.date.updated2021-10-20T23:55:18Z
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2021 Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence (http://creativecommons.org/licenses/by-nc-nd/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited.
gro.hasfulltextFull Text
gro.griffith.authorLam, Alfred K.
gro.griffith.authorGopalan, Vinod
gro.griffith.authorHamid, Faysal-Bin
gro.griffith.authorCheng, Tracie T.


Files in this item

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record