Show simple item record

dc.contributor.authorQiao, B
dc.contributor.authorXu, J
dc.contributor.authorYin, W
dc.contributor.authorXin, W
dc.contributor.authorMa, L
dc.contributor.authorQiao, J
dc.contributor.authorLiu, Y
dc.date.accessioned2021-10-22T05:02:12Z
dc.date.available2021-10-22T05:02:12Z
dc.date.issued2021
dc.identifier.issn0956-5663
dc.identifier.doi10.1016/j.bios.2021.113233
dc.identifier.urihttp://hdl.handle.net/10072/409416
dc.description.abstractHerein, we report a method that combined “aptamer-locker” DNA with CRISPR/Cas12a-based biosensing for sensitive and rapid melamine analysis. Three strategies were harnessed for designing the DNA sensors that were well characterized by circular dichroism (CD) spectroscopy and isothermal titration calorimetry (ITC) in the absence and presence of melamine. The detection parameters were optimized to achieve good analytic performance. As a result, a limit of detection (LOD) as low as 38 nM was achieved, which is below the threshold (1.0 mg/kg) of allowable melamine in infant milk products. In addition, the sensors show high selectivity for melamine against other analogues such as cyanuric acid, ammeline and ammelide. Moreover, our method was effective for rapid melamine analysis in whole milk samples, with or without sample pretreatment, in less than 20 min. Adopting a commercially available portable fluorimeter, on-site analysis of melamine in milk was accomplished. The strategies demonstrated here can expand to detect other non-nucleic-acid targets by simply replacing the aptamers.
dc.description.peerreviewedYes
dc.languageeng
dc.publisherElsevier BV
dc.relation.ispartofpagefrom113233
dc.relation.ispartofjournalBiosensors and Bioelectronics
dc.relation.ispartofvolume183
dc.subject.fieldofresearchAnalytical chemistry
dc.subject.fieldofresearchBiomedical engineering
dc.subject.fieldofresearchNanotechnology
dc.subject.fieldofresearchcode3401
dc.subject.fieldofresearchcode4003
dc.subject.fieldofresearchcode4018
dc.subject.keywordsAptamer
dc.subject.keywordsBiosensor
dc.subject.keywordsCRISPR/Cas12a
dc.subject.keywordsMelamine
dc.subject.keywordsOn-site test
dc.title“Aptamer-locker” DNA coupling with CRISPR/Cas12a-guided biosensing for high-efficiency melamine analysis
dc.typeJournal article
dc.type.descriptionC1 - Articles
dcterms.bibliographicCitationQiao, B; Xu, J; Yin, W; Xin, W; Ma, L; Qiao, J; Liu, Y, “Aptamer-locker” DNA coupling with CRISPR/Cas12a-guided biosensing for high-efficiency melamine analysis, Biosensors and Bioelectronics, 2021, 183, pp. 113233
dcterms.dateAccepted2021-04-05
dc.date.updated2021-10-22T04:52:45Z
gro.hasfulltextNo Full Text
gro.griffith.authorQiao, Bin


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record