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  • High content, multi-parameter analyses in buccal cells to identify Alzheimer's disease

    Author(s)
    Francois, Maxime
    Fenech, Michael F
    Thomas, Philip
    Hor, Maryam
    Rembach, Alan
    Martins, Ralph N
    Rainey-Smith, Stephanie R
    Masters, Colin L
    Ames, David
    Rowe, Christopher C
    Macaulay, S Lance
    Hill, Andrew F
    Leifert, Wayne R
    The Australian Imaging, Biomarkers and Lifestyle Study Research Group
    Griffith University Author(s)
    Zhang, Ping
    Year published
    2016
    Metadata
    Show full item record
    Abstract
    Alzheimer’s disease (AD) is a degenerative brain disorder and is the most common form of dementia. Minimally invasive approaches are required that combine biomarkers to identify individuals who are at risk of developing mild cognitive impairment (MCI) and AD, to appropriately target clinical trials for therapeutic discovery as well as lifestyle strategies aimed at prevention. Buccal mucosa cells from the Australian Imaging, Biomarkers and Lifestyle Flagship Study of Ageing cohort (n=60) were investigated for cytological markers that could be used to identify both MCI and AD individuals. Visual scoring of the buccal cytome ...
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    Alzheimer’s disease (AD) is a degenerative brain disorder and is the most common form of dementia. Minimally invasive approaches are required that combine biomarkers to identify individuals who are at risk of developing mild cognitive impairment (MCI) and AD, to appropriately target clinical trials for therapeutic discovery as well as lifestyle strategies aimed at prevention. Buccal mucosa cells from the Australian Imaging, Biomarkers and Lifestyle Flagship Study of Ageing cohort (n=60) were investigated for cytological markers that could be used to identify both MCI and AD individuals. Visual scoring of the buccal cytome demonstrated a significantly lower frequency of basal and karyorrhectic cells in the MCI group compared with controls. A high content, automated assay was developed using laser scanning cytometry to simultaneously measure cell types, nuclear DNA content and aneuploidy, neutral lipid content, putative Tau and amyloid-β (Aβ) in buccal cells. DNA content, aneuploidy, neutral lipids and Tau were similar in all groups. However, there was significantly lower Tau protein in both basal and karyolytic buccal cell types compared with differentiated buccal cells. Aβ, as measured by frequency of cells containing Aβ signal, as well as area and integral of Aβ signal, was significantly higher in the AD group compared with the control group. Buccal cell Aβ was correlated with mini-mental state examination (MMSE) scores (r = -0.436, P=0.001) and several blood-based biomarkers. Combining newly identified biomarkers from buccal cells with those already established may offer a potential route for more specific biomarker panels which may substantially increase the likelihood of better predictive markers for earlier diagnosis of AD.
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    Journal Title
    Current Alzheimer Research
    Volume
    13
    Issue
    7
    DOI
    https://doi.org/10.2174/1567205013666160315112151
    Subject
    Clinical sciences
    Neurosciences
    Science & Technology
    Life Sciences & Biomedicine
    Clinical Neurology
    Neurosciences
    Neurosciences & Neurology
    Publication URI
    http://hdl.handle.net/10072/409466
    Collection
    • Journal articles

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